Abstract
Changes in secondary structure of DNA and non-histone chromosomal protein HMGB1 during the formation of the complex have been studied by circular dichroism and UV spectroscopy. It was demonstrated that the HMGB1 protein is able to change its secondary structure upon binding to DNA. Based on the assumption that there are two spectroscopically distinguishable forms of the HMGB1 in solution, we estimated the fraction of bound protein. The fraction of bound protein decreases at higher protein to DNA ratios r from 0.48 at r = 0.13 to 0.06 at r = 2.43. It was shown that HMGB1 is able to induce considerable changes in DNA structure, even when the amount of protein actually bound is low.
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Bianchi, M.E. and Manfredi, A., Chromatin and Cell Death, Biochim. Biophys. Acta, 2004, vol. 1677, pp. 181–186.
Bustin, M. and Reeves, R., High-Mobility-Group Chromosomal Proteins: Architectural Components That Facilitate Chromatin Function, Proc. Natl. Acad. Sci. USA, 1996, vol. 54, pp. 35–100.
Chikhirzhina, E.V. and Vorob’ev, V.I., Linker Histones: Conformational Changes and the Role in the Structural Organization of Chromatin, Tsitologiia, 2002, vol. 44, no. 8, pp. 721–736.
Chikhirzhina, E.V., Kostyleva, E.I., Ramm, E.I., and Vorob’ev, V.I., Chromatin Compactification Using a Model System of DNA-Protein Complexes, Tsitologiia, 1998, vol. 40, no. 10, pp. 883–888.
Chikhirzhina, E.V., Polyanichko, A.M., Skvortsov, A.N., Kostyleva, E.I., Houssier, C., and Vorob’ev, V.I., HMG1 Domains: The Victims of the Circumstances, Mol. Biol. (Moscow), 2002, vol. 36, no. 3, pp. 525–531 [Mol. Biol. (Engl. Transl.), 2002, vol. 36, no. 3, pp. 412–418].
Greenfield, N. and Fasman, G.D., Computed Circular Dichroism Spectra for the Evaluation of Protein Conformation, Biochemistry, 1969, vol. 8, pp. 4108–4116.
Ivanov, V.I., Minchenkova, L.E., Schyolkina, A.K., and Poletayev, A.I., Different Conformations of Double-Stranded Nucleic Acid in Solution As Revealed by Circular Dichroism, Biopolymers, 1973, vol. 12, pp. 89–110.
Jamieson, E.R. and Lippard, S.J., Structure, Recognition and Processing of Cisplatin-DNA Adducts, Chem. Rev., 1999, vol. 99, pp. 2467–2498.
Jamieson, E.R., Jacobson, M.P., Barnes, C.M., Chow, C.S., and Lippard, S.J., Structural and Kinetic Studies of a Cisplatin-Modified DNA Icosamer Binding to HMGB1 Domain B, J. Biol. Chem., 1999, vol. 274, pp. 12346–12354.
Kohlstaedt, L.A. and Cole, R.D., Specific Interaction between H1 Histone and High Mobility Protein HMG1, Biochemistry, 1994, vol. 33, pp. 570–575.
Laemmli, U.K., Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
Miles, A.J. and Wallase, B.A., Synchrotron Radiation Circular Dichroism Spectroscopy of Proteins and Applications in Structural and Functional Genomics, Chem. Soc. Rev., 2006, vol. 35, pp. 39–51.
Morrow, J.A., Segall, M.L., Lund-Katz, S., Phillips, M.C., Knapp, M., Rupp, B., and Weisgraber, K.H., Differences in Stability Among the Human Apolipoprotein E Isoforms Determined by the Amino-Terminal Domain, Biochemistry, 2000, vol. 39, pp. 11657–11666.
Palumbo, R., Sampaolesi, M., De Marchis, F., Tonlorenzi, R., Colombetti, S., Mondino, A., Cossu, G., and Bianchi, M.E., Extracellular HMGB1, a Signal of Tissue Damage, Induces Mesoangioblast Migration and Proliferation, J. Cell Biol., 2004, vol. 164, pp. 441–449.
Polyanichko, A.M., Chikhirzhina, E.V., Skvortsov, A.N., Kostyleva, E. I., Colson, P., Houssier, C., and Vorob’ev, V.I., The HMG1 Ta(i)le, J. Biomol. Struct. Dyn., 2002, vol. 19, pp. 1053–1062.
Polyanichko, A.M., Leonenko, Z.V., Cramb, D., Wieser, G., Vorob’ev, V.I., and Chikhirzhina, E.V., Visualization of DNA Complexes with HMGB1 and Its C-Truncated Form HMGB1(A+B), Biofizika, 2008, vol. 53, no. 3, pp. 407–416 [Biophysics (Engl. Transl.), 2008, vol. 53, no. 3, pp. 202–206].
Ramm, E.I., Birshtein, T.M., Bolotina, I.A., Vol’kenshtein, M.V., Vorob’ev, V.I., Dmitrenko, L.M., and Nekrasova, T.N., Histone Structure Study, Mol. Biol. (Moscow), 1970, vol. 4, no. 1, pp. 118–123.
Ramm, E.I., Chikhirzhina, E.V., Kostyleva, E.I., and Vorob’ev, V.I., Conformational Features of Linker Proteins of Supercompact Chromatin from Marine Invertebrate Sperm, Biokhimiya, 1995, vol. 60, no. 1, pp. 150–158.
Ramm, E.I., Vorob’ev, V.I., Birshtein, T.M., Bolotina, I.A., and Volkenshtein, M.V., Circular Dichroism of DNA and Histones in the Free State and in Deoxyribonucleoprotein, Eur. J. Biochem., 1972, vol. 25, pp. 245–253.
Saenger, W., Principles of Nucleic Acid Structure, New York: Springer, 1984. Translated under the title Printsipy strukturnoi organizatsii nukleinovykh kislot, Moscow: Mir, 1987.
Saito, K., Kikuchi, T., Shirakawa, H., and Yoshida, M., The Stabilized Structural Array of Two HMG1/2-Boxes Endowed by a Linker Sequence Between Them Is Requisite for the Effective Binding of HMG1 with DNA, J. Biochem., 1999, vol. 125, pp. 399–405.
Stros, M., HMGB Proteins: Interactions with DNA and Chromatin, Biochem. Biophys. Acta, 2010, vol. 1799, pp. 101–113.
Stros, M., Stokrova, J., and Thomas, J.O., DNA Looping by the HMG-Box Domains of HMG1 and Modulation of DNA Binding by the Acidic C-Terminal Domain, Nucl. Acids Res., 1994, vol. 22, pp. 1044–1051.
Sutrias-Grau, M., Bianchi, M.E., and Bernués, J., High Mobility Group Protein 1 Interacts Specifically with the Core Domain of Human TATA bOx-Binding Protein and Interferes with Transcription Factor IIB within the Pre-initiation Complex, J. Biol. Chem., 1999, vol. 274, pp. 1628–1634.
Travers, A. and Thomas, J.O., Chromosomal HMG-Box Proteins, in Chromatin Structure and Dynamics: State-of-the-Art, New York: Elsevier, B. V., 2004, pp. 103–126.
Vellyuz, L., Legran, M., and Grozhan, M., Opticheskii krugovoi dikhroizm (Optical Circular Dichroism), Moscow: Mir, 1967.
Wang, D. and Lippard, S.J., Cellular Processing of Platinum Anticancer Drugs, Nat. Rev. Drug Discov., 2005, vol. 4, pp. 307–320.
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Original Russian Text © A.M. Polyanichko, T.J. Rodionova, V.I. Vorob’ev, E.V. Chikhirzhina, 2011, published in Tsitologiya, Vol. 53, No. 1, 2011, pp. 55–60.
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Polyanichko, A.M., Rodionova, T.J., Vorob’ev, V.I. et al. Conformational properties of nuclear protein HMGB1 and specificity of its interaction with DNA. Cell Tiss. Biol. 5, 114–119 (2011). https://doi.org/10.1134/S1990519X1102012X
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DOI: https://doi.org/10.1134/S1990519X1102012X