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Proteins of the Akt1/GSK-3β signaling pathway in peripheral blood mononuclear cells of patients with affective disorders

  • Clinical Neurochemistry
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Abstract

The Akt1/GSK-3β signaling pathway is involved in the regulation of biological processes in nerve cells. Here we studied the intracellular proteins Akt1 and GSK-3β and their phosphoforms in patients with affective disorders. Sixty patients with diagnoses of depressive episodes, major depressive disorder, and bipolar affective disorder (BAD) and 34 mentally and somatically healthy subjects were examined. Proteins of the Akt1/GSK-3β signaling pathway (total glycogen synthase kinase 3β (GSK-3β), phospho-serine-9-GSK-3β, total protein kinase Akt1, phospho-serine-473-Akt1) were measured in peripheral blood mononuclear cells using the immunoblotting technique. High levels of total GSK-3β were found in groups of patients with affective disorders. Patients with recurrent depressive disorder had an increased level of total GSK-3β as compared with the level in depressive episode. The level of total Akt1 in patients decreased as compared with the control group. Patients with depressive disorder had decreased levels of phospho-serine-473-Akt1. Our results confirm the hypothesis of the involvement of glycogen synthase kinase-3β in the pathogenesis of affective disorders.

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References

  1. Untsun, T.B. and Sartorius, N., Mental Illness in General Health Care: An International Study. John Wiley and Sons, 1995, p. 398.

    Google Scholar 

  2. Krasnov, V.N., Rasstroistva affektivnogo spektra (Affective disorders), Moscow: Prakticheskaya meditsina, 2011.

    Google Scholar 

  3. Tiganov, A.S., Kopeiko, G.I., Brusov, O.S., and Klyushnik, T.P., Zh. Nevrol. Psikhiatr. Im. S.S. Korsakova, 2012, vol. 112, no. 11, pp. 65–72.

    CAS  PubMed  Google Scholar 

  4. Vyalova, N.M., Fedorenko, O.Yu., Losenkov, I.S., Simutkin, G.G., Ivanova, S.A., and Bokhan, N.A., Fundamental Research, 2013, no. 12, part 1, pp. 107–110.

    Google Scholar 

  5. Losenkov, I.S., Vyalova, N.M., Simutkin, G.G., Ivanova, S.A., and Bokhan, N.A., Sovr. Probl. Nauki i Obrazov., 2013, no. 6, http://www.science-education.ru/113-11027.

    Google Scholar 

  6. Fedorenko, O., Strutz-Seebohm, N., Henrion, U., Ureche, O.N., Seebohm, G., and Lang, U.E., Psychopharmacology, 2008, vol. 199, no. 1, pp. 47–54.

    Article  CAS  PubMed  Google Scholar 

  7. Fedorenko, O., Tang, C., Sopjani, M., Foller, M., Gehring, E.M., Strutz-Seebohm, N., Ureche, O.N., Lang, F., Seebohm, G., Ivanova, S., Semke, A., and Lang, U.E., Psychopharmacology, 2009, vol. 206, no. 3, pp. 429–435.

    Article  CAS  PubMed  Google Scholar 

  8. Ivanova, S.A., Fedorenko, O.Yu., Smirnova, L.P., and Semke, A.V., Sib. Vestn. Psykhitrii i Narkologii, 2013, no. 1, pp. 12–16.

    Google Scholar 

  9. Dunman, S.R. and Voleti, B., Trends Neurosci., 2013, vol. 35, no. 1, pp. 47–56.

    Article  Google Scholar 

  10. Beaulieu, J.M., J. Psychiatry Neurosci., 2011, vol. 37, no. 1, pp. 9–16.

    Google Scholar 

  11. Rayasam, G.V., Tulasi, V.K., Sodhi, R., Davis, J.A., and Ray, A., Br. J. Pharmacol., 2009, vol. 156, no. 6, pp. 885–898.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  12. Ivanova, S.A., Semke, V.Ya., Vetlugina, T.P., Rakitina, N.M., Kudyakova, T.A., and Simutkin, G.G., Neurosci. Behav. Physiol., 2007, vol. ò. 37, no. 5, pp. 527–530.

    Article  Google Scholar 

  13. Klein, P.S. and Melton, D.A., Proc. Natl. Acad. Sci. USA, 1996, vol. 93, no. 16, pp. 8455–8459.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  14. Li, X. and Jope, R.S., Neuropsychopharmacology, 2010, vol. 35, no. 11, pp. 2143–2154.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Li, X., Friedman, A.B., Roh, M.S., and Jope, R.S., J. Neurochem., 2005, vol. 92, no. 3, pp. 701–704.

    Article  PubMed Central  PubMed  Google Scholar 

  16. Vetlugina, T.P., Ivanova, S.A., Semke, V.Ya., and Kornetov, N.A., Byull. Eksp. Biol. Med., 2000, vol. 129.

  17. Gladkevich, A., Kauffman, H.F., and Korf, J., Prog. Neuropsychopharmacol. Biol. Psychiatry, 2004, vol. 28, no. 3, pp. 559–576.

    Article  PubMed  Google Scholar 

  18. Freland, L. and Beaulieu, J.M., Front. Mol. Neurosci., 2012, vol. 5, pp. 9–15.

    Google Scholar 

  19. Natvig, D.B., Limfotsity: vydelenie, fraktsionirovanie, kharakteristika (Lymphocytes: Isolation, Fractionation and Characterization), Moscow: Bukinist, 1980.

    Google Scholar 

  20. Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

  21. Merikangas, K.R. and Kalaydjian, A., Curr. Opin. Psychiatry, 2007, vol. 20, no. 4, pp. 353–358.

    Article  PubMed  Google Scholar 

  22. Simutkin, G.G., Obozrenie Psikhiatrii i Meditsinskoi Psikhologii im. V.M. Bekhtereva, 2010, no. 1, pp. 10–14.

    Google Scholar 

  23. Emamian, E.S., Birnbaum, M.J., Karayiorgou, M., and Gogos, J.A., Nat. Genet., 2004, vol. 36, no. 2, pp. 131–137.

    Article  CAS  PubMed  Google Scholar 

  24. Uzbekov, V.G., Misionzhnik, E.Y., Maximova, N.M., and Vertogradova, O.P., Hum. Psychopharmacol. Clin. Exp., vol. 21, pp. 109–115.

  25. Manning, B.D. and Cantley, L.C., Cell, 2004, vol. 129, no. 7, pp. 1261–1247.

    Article  Google Scholar 

  26. Belmaker, R.H. and Agam, A., N. Engl. J. Med., 2008, vol. 358, pp. 55–68.

    Article  CAS  PubMed  Google Scholar 

  27. Karege, F., Perroud, N., Burkhardt, S., Schwald, M., Ballmann, E., La Harpe, R., and Malafosse, A, Biol. Psychiatry, 2007, vol. 61, no. 2, pp. 240–245.

    Article  CAS  PubMed  Google Scholar 

  28. Li, X., Friedman, A.B., Zhu, W., Wang, L., Boswell, S., May, R.S., Davis, L.L., and Jope, R.S., Biol. Psychiatry, 2007, vol. 61, no. 2, pp. 216–222.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. Pandey, G.N., Ren, X., Rizavi, H.S., and Dwivedi, Y., J. Psychiatr. Res., vol. 44, no. 3, pp. 143–148.

  30. Polter, A.M., Beurel, E., Yang, S., Garner, R., Song, L., Miller, C.A., Sweatt, J.D., McMahon, L., Bartolucci, A.A., Li, X., and Jope, R.S., Neuropsychopharmacology, 2010, vol. 35, no. 8, pp. 1761–1774.

    CAS  PubMed Central  PubMed  Google Scholar 

  31. Kozlovsky, N., Amar, S., Belmaker, R.H., and Agam, G., Int. J. Neuropsychopharmacol., 2006, vol. 9, no. 3, pp. 337–342.

    Article  CAS  PubMed  Google Scholar 

  32. Chen, G., Huang, L.D., Jiang, Y.M., and Manji, H.K., J. Neurochem, 1999, vol. 72, no. 3, pp. 1327–1330.

    Article  CAS  PubMed  Google Scholar 

  33. Dunlop, B.W. and Nemeroff, C.B., Arch. Gen. Psychiatry, 2007, vol. 64, no. 3, pp. 327–337.

    Article  CAS  PubMed  Google Scholar 

  34. Li, X., Zhu, W., Roh, M.S., Friedman, A.B., Rosborough, K., and Jope, R.S., Neuropsychopharmacology, 2004, vol. 29, no. 8, pp. 1426–1431.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  35. Thornton, T.M., Pedraza-Alva, G., Deng, B., Wood, C.D., Aronshtam, A., Clements, J.L., Sabio, G., Davis, R.J., Matthews, D.E., Doble, B., and Rincon, M., Science, 2008, vol. 230, no. 5876, pp. 667–670.

    Article  Google Scholar 

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Authors and Affiliations

  1. Mental Health Research Institute, Siberian Branch, Russian Academy of Medical Sciences, Tomsk, Russia

    I. S. Losenkov, S. A. Ivanova, N. A. Vyalova, G. G. Simutkin & N. A. Bokhan

  2. ul. Aleutskaya, 4, Tomsk, 634014, Russia

    I. S. Losenkov

Authors
  1. I. S. Losenkov
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  2. S. A. Ivanova
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  3. N. A. Vyalova
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  4. G. G. Simutkin
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  5. N. A. Bokhan
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Correspondence to I. S. Losenkov.

Additional information

Original Russian Text © I.S. Losenkov, S.A. Ivanova, N.A. Vyalova, G.G. Simutkin, N.A. Bokhan, 2014, published in Neirokhimiya, 2014, Vol. 31, No. 3, pp. 240–245.

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Losenkov, I.S., Ivanova, S.A., Vyalova, N.A. et al. Proteins of the Akt1/GSK-3β signaling pathway in peripheral blood mononuclear cells of patients with affective disorders. Neurochem. J. 8, 208–213 (2014). https://doi.org/10.1134/S1819712414030106

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  • DOI: https://doi.org/10.1134/S1819712414030106

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