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Involvement of the Arp2/3 complex and WASP proteins in the effect of glutoxim and molixan on intracellular Ca2+ concentration in macrophages

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

The Fura-2AM fluorescent Ca2+ probe was used to study the possibility that the Arp2/3 complex and WASP proteins are involved in the effects of glutoxim and molixan on the intracellular Ca2+ concentration in macrophages. It has been demonstrated that preincubation of macrophages with inhibitors of the Arp2/3 complex or WASP proteins (CK-0944666 or wiskostatin, respectively) results in a significant suppression of Ca2+-responses induced by glutoxim or molixan. This suggests that polymerization of actin filaments is a process involved in the effect of glutoxim or molixan on intracellular Ca2+ concentration in macrophages.

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References

  1. Borisov, A.E., Kozhemyakin, L.A., Antushevich, A.E., et al., Vestn. Hirurgii im. I.I. Grekova, 2001, vol. 4, pp. 32–38.

    Google Scholar 

  2. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Cell Tissue Biol., 2008, vol. 2, pp. 322–332.

    Article  Google Scholar 

  3. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Tsitologiya, 2011, vol. 53, no. 9, p. 708.

    Google Scholar 

  4. Krutetskaya, Z.I., Lebedev, O.E., Kurilova, L.S., et al., Dokl. Biol. Sci., 2008, vol. 422, no. 4, pp. 296–297.

    Article  CAS  PubMed  Google Scholar 

  5. Krutetskaya, Z.I., Lebedev, O.E., Kurilova, L.S., et al., Dokl. Biol. Sci., 2009, vol. 428, no. 2, pp. 407–409.

    Article  CAS  PubMed  Google Scholar 

  6. Kurilova, L.S., Krutetskaya, Z.I., Lebedev, O.E., et al., Tsitologiya, 2012, vol. 54, no. 2, pp. 135–142.

    CAS  Google Scholar 

  7. Krutetskaya, Z.I., Kurilova, L.S., Antonov, V.G., et al., Dokl. Biol. Sci., 2013, vol. 451, no. 3, pp. 193–195.

    Article  CAS  PubMed  Google Scholar 

  8. Krutetskaya, Z.I., Kurilova, L.S., Naumova, A.A., et al., Dokl. Biol. Sci., 2014, vol. 457, no. 2, pp. 252–254.

    Article  CAS  PubMed  Google Scholar 

  9. Xu, X.P., Rouiller, I., Slaughter, B.D., et al., EMBO J., 2012, vol. 31, pp. 236–247.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Bouma, G., Burns, S.O., and Thrasher, A.J., Immunobiology, 2009, vol. 214, pp. 778–790.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Grynkiewicz, G., Poenie, M., and Tsien, R.Y., J. Biol. Chem., 1985, vol. 260, pp. 3440–3450.

    CAS  PubMed  Google Scholar 

  12. Nolen, B.J., Tomasevic, N., Russel, A., et al., Nature, 2009, vol. 460, pp. 1031–1034.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Peterson, J.R., Bickford, L.S., Morgan, D., et al., Nat. Struct. Mol. Biol., 2004, vol. 11, pp. 747–755.

    Article  CAS  PubMed  Google Scholar 

  14. Kurilova, L.S., Krutetskaya, Z.I., and Lebedev, O.E., Tsitologiya, 2006, vol. 48, no. 10, pp. 867–874.

    CAS  Google Scholar 

  15. Rosado, J.A. and Sage, S.O., J. Physiol., 2000, vol. 526, no. 2, pp. 221–229.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. St. Petersburg State University, St. Petersburg, 199034, Russia

    Z. I. Krutetskaya, L. S. Milenina, A. A. Naumova, S. N. Butov, V. G. Antonov & A. D. Nozdrachev

Authors
  1. Z. I. Krutetskaya
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  2. L. S. Milenina
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  3. A. A. Naumova
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  4. S. N. Butov
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  5. V. G. Antonov
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  6. A. D. Nozdrachev
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Correspondence to Z. I. Krutetskaya.

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Original Russian Text © Z.I. Krutetskaya, L.S. Milenina, A.A. Naumova, S.N. Butov, V.G. Antonov, A.D. Nozdrachev, 2015, published in Doklady Akademii Nauk, 2015, Vol. 464, No. 2, pp. 227–230.

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Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Involvement of the Arp2/3 complex and WASP proteins in the effect of glutoxim and molixan on intracellular Ca2+ concentration in macrophages. Dokl Biochem Biophys 464, 279–282 (2015). https://doi.org/10.1134/S1607672955050013

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  • DOI: https://doi.org/10.1134/S1607672955050013

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