Skip to main content
SpringerLink
Log in

Radioprotective Effect of Nanocerium by Irradiation of Mice with Carbon Ions in Medium and Lethal Doses

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

The data of the study of the radioprotective properties of nanocerium (nCeO2) after total irradiation of mice with carbon ions in medium and lethal doses according to the micronucleus test and the criterion of 30-day survival are presented. A significant protective effect of nCeO2 upon irradiation at medium doses was observed at per os administration for 5 days before irradiation (that is, at long-term prophylactic use). Mouse survival data showed no protective effect of per os administration of nCeO2 in contrast to the micronucleus test results. After injections of both nCeO2 and saline solution 24 h before or immediately after irradiation, the radioprotective effect was detected using both methods. The data obtained revealed the dependence of the observed effects on the mode and time of nCeO2 administration, the influence of the solvent, the level of doses and the quality of radiation, as well as demonstrated the possibility of using nanocerium preparations to protect organisms from radiation with high LET values and the importance of further studies of the radioprotective properties of new nanomaterials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

REFERENCES

  1. Tinganelli, W. and Durante, M., Carbon ion radiobiology, Cancers (Basel), 2020, vol. 12, no. 10, p. 3022.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Grau, C., Durante, M., Georg, D., et al., Particle therapy in Europe, Mol. Oncol., 2020, vol. 14, no. 7, pp. 1492–1499.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Seidensaal, K., Mattke, M., Haufe, S., et al., The role of combined ion-beam radiotherapy (cibrt) with protons and carbon ions in a multimodal treatment strategy of inoperable osteosarcoma, Radiother. Oncol., 2021, vol. 159, pp. 8–16.

    Article  CAS  PubMed  Google Scholar 

  4. Thomas, H. and Timmermann, B., Paediatric proton therapy, Br. J. Radiol., 2020, vol. 93, no. 1107, p. 20190601.

    Article  PubMed  PubMed Central  Google Scholar 

  5. Cortese, F., Klokov, D., Osipov, A., et al., Vive la radiorésistance!: converging research in radiobiology and biogerontology to enhance human radioresistance for deep space exploration and colonization, Oncotarget, 2018, vol. 9, no. 18, pp. 14692–14722.

    Article  PubMed  PubMed Central  Google Scholar 

  6. Vasin, M.V., Classification of anti-radiation agents as a reflection of the current state and prospects for the development of radiation pharmacology, Radiats. Biol. Radioekol., 2013, vol. 53, no. 5, pp. 459–467.

    CAS  Google Scholar 

  7. Xie, J., Wang, C., Zhao, F., et al., Application of multifunctional nanomaterials in radioprotection of healthy tissues, Adv. Healthc. Mater., 2018, vol. 7, no. 20, p. 1800421.

    Article  Google Scholar 

  8. Stephen, InbarajB. and Chen, B.H., An overview on recent in vivo biological application of cerium oxide nanoparticles, Asian J. Pharm. Sci., 2020, vol. 15, no. 5, pp. 558–575.

  9. Colon, J., Herrera, L., Smith, J., et al., Protection from radiation-induced pneumonitis using cerium oxide nanoparticles, Nanomedicine, 2009, vol. 5, no. 2, pp. 225–231.

    Article  CAS  PubMed  Google Scholar 

  10. Yokel, R.A., Tseng, M.T., Butterfield, D.A., et al., Nanoceria distribution and effects are mouse-strain dependent, Nanotoxicology, 2020, vol. 14, no. 6, pp. 827–846.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Carlander, U., Moto, T.P., Desalegn, A.A., et al., Physiologically based pharmacokinetic modeling of nanoceria systemic distribution in rats suggests dose- and route-dependent biokinetics, Int. J. Nanomed., 2018, vol. 13, pp. 2631–2646.

    Article  CAS  Google Scholar 

  12. Popova, N.R., Popov, A.L., Ermakov, A.M., et al., Ceria-containing hybrid multilayered microcapsules for enhanced cellular internalisation with high radioprotection efficiency, Molecules, 2020, vol. 25, no. 13, p. 2957.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Popov, A.L., Zaichkina, S.I., Popova, N.R., et al., Radioprotective effects of ultra-small citrate-stabilized cerium oxide nanoparticles in vitro and in vivo, RSC Adv., 2016, vol. 6, no. 108, pp. 106141–106149.

    Article  CAS  Google Scholar 

  14. Smith, J.A., Broek, F.A., Martorell, J.C., et al., Principles and practice in ethical review of animal experiments across Europe: summary of the report of the FELASA working group on ethical evaluation of animal experiments, Lab. Anim., 2007, vol. 41, no. 2, pp. 143–160.

    Article  PubMed  Google Scholar 

  15. Ivanova, O.S., Ivanov, V.K., Shcherbakov, A.B., et al., One-stage synthesis of ceria colloid solutions for biomedical use, Dokl. Chem., 2011, vol. 437, no. 2, pp. 103–106.

    Article  CAS  Google Scholar 

  16. Durnev, A.D., Arzamastsev E.V., Bukhman, V.M., et al., Features of the study of the general toxic effect of drugs and medical devices containing nanoparticles, in Rukovodstvo po provedeniyu klinicheskikh issledovanii lekarstvennykh sredstv (Guidelines for Conducting Clinical Trials of Drugs), Mironov A.N., Ed., Moscow: Grif i K, 2012, pp. 25–34.

  17. Zaichkina, S.I., Kondakova, N.V., Rozanova, O.M., et al., Testing the radioprotective effect of biologically active substances in the range of medium and low doses of radiation using a cytogenetic indicator—micronucleus test, Khim.-Farm. Zh., 2004, vol. 38, no. 8, pp. 405–410.

    CAS  Google Scholar 

  18. Vasin, M.V., Protivoluchevye lekarstvennye sredstva (Radioprotective Drugs), Moscow: Ross. Med. Akad. Poslediplomnogo Obrazovaniya, 2010.

  19. Head, K., Snidvongs, K., Glew, S., et al., Saline irrigation for allergic rhinitis, Cochrane Database Syst. Rev., 2018, vol. 6, no. 6, p. CD012597.

    PubMed  Google Scholar 

  20. Kazimirova, A., Baranokova, M., Staruchova, M., et al., Titanium dioxide nanoparticles tested for genotoxicity with the comet and micronucleus assays in vitro, ex vivo and in vivo, Mutat. Res. Genet. Toxicol. Environ. Mutagen., 2019, vol. 843, pp. 57–65.

    Article  CAS  PubMed  Google Scholar 

Download references

ACKNOWLEDGMENTS

We express our sincere gratitude to A.L. Popov, Head of the Laboratory of Cell and Tissue Growth, Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, for providing the nanocrystalline cerium oxide sample and interest in our research.

Funding

The study of the radioprotective effect of nanocerium upon irradiation of mice with medium and lethal doses of carbon ions was performed as part of fundamental scientific research on topics that correspond to the state assignment no. 075-00381-21-00 (Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences). The study was also supported in part by the R&D agreement with the Physical-Technical Center, Lebedev Physical Institute, no. 07/21.

Author information

Authors and Affiliations

  1. Branch “Physical-Technical Center” of P.N. Lebedev Physical Institute, Russian Academy of Sciences, Protvino, Russia

    V. E. Balakin, T. A. Belyakova, N. S. Strelnikova & A. V. Smirnov

  2. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia

    O. M. Rozanova & E. N. Smirnova

  3. Institute for High Energy Physics named by A.A. Logunov, National Research Centre “Kurchatov Institute”, Protvino, Russia

    A. G. Vasilyeva

Authors
  1. V. E. Balakin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. M. Rozanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. N. Smirnova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. A. Belyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. S. Strelnikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. V. Smirnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. G. Vasilyeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. A. Belyakova.

Ethics declarations

Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. All applicable international, national and institutional guidelines for the care and use of animals in the performance of work have been observed. The studies were carried out in accordance with the requirements of the Federation of European Scientific Associations for the maintenance and use of laboratory animals in scientific research (Federation of European Laboratory Animal Science Association, FELASA),and the experimental plan was approved by the Commission on Biological Safety and Bioethics of the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences (protocol no. 26/2021 dated February 9, 2021).

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Balakin, V.E., Rozanova, O.M., Smirnova, E.N. et al. Radioprotective Effect of Nanocerium by Irradiation of Mice with Carbon Ions in Medium and Lethal Doses. Dokl Biochem Biophys 507, 283–288 (2022). https://doi.org/10.1134/S1607672922060023

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672922060023

Keywords:

Search

Navigation