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Nortriptyline Modulates the Migration of Peripheral Blood Lymphocytes and Monocytes in Patients with Chronic Obstructive Pulmonary Disease

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract—

In the present study, the effect of nortriptyline (1 and 10 μM), budesonide (10 nM) and their combination on the migration of peripheral blood lymphocytes and monocytes from patients with chronic obstructive pulmonary disease (COPD) towards chemokines CCL5 and CXCL10 was evaluated by flow cytometry. Nortriptyline (10 μM), both alone and in combination with budesonide, inhibited the migration of T helper cells, cytotoxic T lymphocytes, NK cells and B lymphocytes towards CCL5 and CXCL10, as well as enhanced monocyte migration towards these chemokines. The combination of nortriptyline (1 μM) and budesonide suppressed the chemotaxis of lymphocyte subpopulations towards CXCL10, but not towards CCL5, more effectively than budesonide alone. The combination of nortriptyline (10 μM) and budesonide inhibited the migration of lymphocyte subpopulations towards CCL5 and CXCL10 and activated monocyte chemotaxis towards both chemokines more effectively than budesonide alone. The results of this study demonstrate the ability of nortriptyline alone to modulate the migration of peripheral blood lymphocytes and monocytes from patients with COPD and to potentiate the effects of budesonide.

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Funding

The study was supported by the Belarusian State Scientific Program “Fundamental and Applied Sciences for Medicine” (task no. 2.56).

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Authors and Affiliations

  1. Belarusian State Medical University, Minsk, Belarus

    A. G. Kadushkin, A. D. Tahanovich, T. S. Kolesnikova & E. V. Khadasouskaya

  2. Republican Scientific and Practical Center for Pediatric Oncology, Hematology and Immunology, Minsk Region, Belarus

    L. V. Movchan & T. V. Shman

Authors
  1. A. G. Kadushkin
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  2. A. D. Tahanovich
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  3. L. V. Movchan
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  4. T. S. Kolesnikova
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  5. E. V. Khadasouskaya
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  6. T. V. Shman
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Corresponding author

Correspondence to A. G. Kadushkin.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. The study was approved by the Biomedical Ethics Committee of Belarusian State Medical University (protocol no. 8, January 21, 2019). Before blood sampling and lung function testing, all subjects gave written informed consent to participate in the study.

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Kadushkin, A.G., Tahanovich, A.D., Movchan, L.V. et al. Nortriptyline Modulates the Migration of Peripheral Blood Lymphocytes and Monocytes in Patients with Chronic Obstructive Pulmonary Disease. Dokl Biochem Biophys 507, 307–311 (2022). https://doi.org/10.1134/S1607672922050076

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  • DOI: https://doi.org/10.1134/S1607672922050076

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