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Haponin (EIF1AD) interacts with glyceraldehyde 3-phosphate dehydrohenase in the CHO-K1 cell line

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Abstract

Haponin (HLDF-alike protein) was previously identified from the human promyelocytic leukemia HL-60 cell line. For the functional study of this protein, we obtained recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system. Antibodies against 6xHis-haponin were produced, and the expression of endogenous haponin was demonstrated in mammalian cell lines of different origin. Using affinity chromatography and immunoprecipitation methods, we have shown that in CHO-K1 cells haponin interacts with glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which is one of the vital glycolytic enzymes with a diverse set of noncanonical functions.

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Abbreviations

eIF1A:

eukaryotic initiation translation factor 1A

eIF1AD:

eukaryotic translation initiation factor 1A domain containing protein

GAPDH:

glyceraldehyde-3-phosphatede-hydrohenase

HLDF:

HL-60 differentiation factor

STAT1:

signal transducer and activator of transcription 1

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    T. V. Rakitina, O. V. Bogatova, E. V. Smirnova, V. I. Pozdeev, I. A. Kostanyan & V. M. Lipkin

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  1. T. V. Rakitina
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  2. O. V. Bogatova
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  3. E. V. Smirnova
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  4. V. I. Pozdeev
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  5. I. A. Kostanyan
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  6. V. M. Lipkin
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Correspondence to T. V. Rakitina.

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Original Russian Text © T.V. Rakitina, O.V. Bogatova, E.V. Smirnova, V.I. Pozdeev, I.A. Kostanyan, V.M. Lipkin, 2010, published in Bioorganicheskaya Khimiya, 2010, Vol. 36, No. 3, pp. 312–318.

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Rakitina, T.V., Bogatova, O.V., Smirnova, E.V. et al. Haponin (EIF1AD) interacts with glyceraldehyde 3-phosphate dehydrohenase in the CHO-K1 cell line. Russ J Bioorg Chem 36, 287–292 (2010). https://doi.org/10.1134/S1068162010030027

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