Abstract
Highly-efficient antiretroviral therapy allows controlling human immunodeficiency virus (HIV) and preventing the development of immunodeficiency. However, the patients who receive therapy may develop different complications, including B-cell lymphomas. One of oncogenesis’ mechanisms in HIV-infected patients is associated with the activity of the viral Tat protein, which is able to penetrate into B-cells. In order to study the effect of the Tat protein on B-cells, a report is given on the production and characterization of the cell lines based on the cultured RPMI 8866 B-cell line demonstrating constant and inducible expression of the Tat protein.
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ACKNOWLEDGMENTS
We are grateful to Eric Kowarz for kindly providing the pSBbi-GP and pSBtet-GP plasmids (Addgene рlasmids #60511 and #60495) and to Zsuzsanna Izsvak for kindly providing the SB100X plasmid (Addgene рlasmid #34879).
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The work was supported by the Russian Science Foundation (grant no. 17-75-20199).
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Conflict of interests. The authors declare that they have no conflict of interests. No experimentation involving animals was part of the present work.
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Translated by E. Martynova
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Gorbacheva, M.A., Tikhomirova, M.A., Potashnikova, D.M. et al. Production of Stable Cell Lines on the Basis of the Cultured RPMI 8866 B-Cells with Constant and Inducible Expression of the Human Immunodeficiency Virus Tat Protein. Russ J Dev Biol 50, 275–280 (2019). https://doi.org/10.1134/S1062360419050060
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DOI: https://doi.org/10.1134/S1062360419050060