Abstract
Expression of genes and heat shock proteins in normal intact retina of the Spanish Ribbed Newt Pleurodeles waltl was studied using polymerase chain reaction, Western blot hybridization, and immunohis-tochemistry. It was shown that the proteins HSP70 and HSP90, as well as their encoding transcripts of relevant genes, are constitutively expressed in eye tissues. These proteins were distributed differentially, and they were characterized by expression of different levels in the retina: HSP70 dominated in the external retina, while HSP90 dominated in the internal one, in particular, in Muller glial cells and the optic nerve. Transcripts and heat shock proteins HSP70 and HSP90 were also found in the retinal pigment epithelium and eye growth zone.
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Original Russian Text © P.P. Avdonin, Yu.V. Markitantova, V.A. Poplinskaya, E.N. Grigoryan, 2013, published in Izvestiya Akademii Nauk, Seriya Biologicheskaya, 2013, No. 4, pp. 389–397.
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Avdonin, P.P., Markitantova, Y.V., Poplinskaya, V.A. et al. Determination of mRNA-transcripts and heat shock proteins HSP70 and HSP90 in the retina of the adult Spanish Ribbed Newt Pleurodeles waltl . Biol Bull Russ Acad Sci 40, 343–350 (2013). https://doi.org/10.1134/S106235901304002X
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DOI: https://doi.org/10.1134/S106235901304002X