Abstract
A new quantification method is proposed for the determination of serum estrone (E1), estradiol (E2), and estriol (E3) based on high-performance liquid chromatography–tandem mass spectrometry combined with differential ion mobility separation (SelexION Technology). This approach allows determining analytes with limits of detection of 4.5, 18 and 45 fg on column for E1, E2, and E3, respectively. The lowest limits of quantification are 0.1, 0.5, and 0.5 pg mL–1 in serum for E1, E2, and E3, respectively. The suitability of proposed method for diagnostic purposes is demonstrated in an analysis of serum from 114 patients of different genders and ages.
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REFERENCES
Serov, V.N., Obstet., Gynecol. Reprod., 2010, vol. 4, no. 1, p. 21.
Olisov, D.A., Chagovets, V.V., Starodubtseva, N.L., et al., Biomed. Chem.: Res. Methods, 2021, vol. 4, no. 2, p. 1.
Bussy, U., Chung-Davidson, Y.-W., Buchinger, T.J., et al., Steroids, 2017, vol. 123, p. 13.
Rice, S.L. and Hale, R.C., Anal. Chem., 2009, vol. 81, p. 6716.
Boelen, A. and Ruiter, A., Claahsen-van der Grinten, H.L., et al., Bioanalysis, 2016, vol. 8, no. 5, p. 375.
Ke, Y., Bertin, J., Gonthier, R., et al., J. Steroid Biochem. Mol. Biol., 2014, vol. 144, p. 523.
Harwood, D.T. and Handelsman, D.J., Clin. Chim. Acta, 2009, vol. 409, p. 78.
Shi, Z., Huai, Q., Li, X., et al., J. Chromatogr. Sci., 2019, vol. 58, p. 171.
Caron, P., Audet-Walsh, E., Lepine, J., et al., Anal. Chem., 2009, vol. 81, p. 10143.
Zhong, Q., Hu, Y., Hu, Y., et al., J. Chromatogr. A, 2012, vol. 1241, p. 13.
Navarrete, A., Martinez-Alcazar, M.P., Duran, I., et al., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2013, vols. 921–922, p. 35.
Taylor, P.J., Clin. Biochem, 2005, vol. 38, p. 328.
Xiao-Yao, X., McCalley, D.V., and McEvoya, J., J. Chromatogr. A, 2001, vol. 923, p. 195.
Nelson, R.E., Grebe, S.K., O’Kane, D.J., et al., Clin. Chem., 2004, vol. 50, no. 2, p. 373.
Keski-Rahkonen, P., Desai, R., Jimenez, M., et al., Anal. Chem., 2015, vol. 87, no. 14, p. 7180.
Xu, L. and Spink, D.C., Anal. Biochem., 2008, vol. 375, p. 105.
Schneider, B.B., Covey, T.R., Coy, S.L., et al., Int. J. Mass Spectrom. Ion Processes, 2010, vol. 298, p. 45.
Ray, J.A., Kushnir, M.M., Yost, R.A., et al., Clin. Chim. Acta, 2015, vol. 438, p. 330.
Fiers, T., Casetta, B., Bernaert, B., et al., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2019, vols. 893–894, p. 57.
ACKNOWLEDGMENTS
The authors are grateful to the staff of the Institute of Pediatric Endocrinology of the Federal State Budgetary Institution National Medical Research Center for Endocrinology of the Ministry of Health of Russia for their help in selecting patient groups for analysis.
Funding
The work was carried out within the framework of the State Task of the Ministry of Health of the Russian Federation no. 121030100030-3.
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Translated by V. Kudrinskaya
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Ioutsi, V.A., Panov, Y.M., Usol’tseva, L.O. et al. Analysis of Serum Estrogens Using High-Performance Liquid Chromatography–Tandem Mass Spectrometry Coupled to Differential Ion Mobility Spectrometry. J Anal Chem 77, 1760–1766 (2022). https://doi.org/10.1134/S1061934822140027
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DOI: https://doi.org/10.1134/S1061934822140027