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Determination of Melatonin Hormone and Neurotransmitters in Cerebrospinal Fluid: Method Validation and Uncertainty Calculations

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Abstract

The uncertainty of measurement for well-known neurotransmitters like serotonin, noradrenalin and gamma-aminobutyric acid (GABA), glutamate and melatonin hormone with high performance liquid chromatography−fluorescence detection was calculated after the method validation. Two methods were developed for the determination of the neurotransmitters. A derivatization step was performed for the determination of GABA and glutamate. Sensitivity, method detection limit, limit of quantification, linearity, recovery, interday and intraday precision values were calculated. Low detection limit values were obtained especially for the determination of GABA and glutamate. Then, bottom-up approach was used to calculate measurement uncertainty. The critical stages of the method were evaluated. The major sources of the uncertainty budget were calibration curves, stock solution and recovery. The calculated percentage relative uncertainty values for the compounds changed between 10.1 and 16.7.

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References

  1. Siegel, A. and Sapru, H.N., Essential Neuroscience, Baltimore: Lippincott Williams & Wilkins, 2006, 2nd ed.

    Google Scholar 

  2. Suszkiw, J.B., Cell Physiology Sourcebook: A Molecular Approach, Chapter 41: Synaptic Transmission, New York: Academic, 2001, 3rd ed.

    Google Scholar 

  3. Shah, A.J., Crespi, F., and Heidbreder, C., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2002, vol. 781, p. 151.

    Article  CAS  Google Scholar 

  4. Van der Zeyden, M., Oldenziel, W.H., Rea, K., Cremers, T.I., and Westerink, B.H., Pharmacol., Biochem. Behav., 2008, vol. 90, p. 135.

    Article  CAS  Google Scholar 

  5. de Freitas Silva, D.M., Ferraz, V.P., and Ribeiro, A.M., J. Neurosci. Methods, 2009, vol. 177, p. 289.

    Article  CAS  PubMed  Google Scholar 

  6. Perry, M., Li, Q., and Kennedy, R.T., Anal. Chim. Acta, 2009, vol. 653, p. 1.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Ishida, J., Yoshitake, T., Fujino, K., Kawano, K., Kehr, J., and Yamaguchi, M., Anal. Chim. Acta, 1998, vol. 365, p. 227.

    Article  CAS  Google Scholar 

  8. Mishra, A., Upadhyay, A., Patra, A., Chaudhury, S., and Chattopadhyay, P., Sci. Pharm., 2009, vol. 77, p. 367.

    Article  CAS  Google Scholar 

  9. Hamase, K., Hirano, J., Kosai, Y., Tomita, T., and Zaitsu, K., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2004, vol. 811, p. 237.

    Article  CAS  Google Scholar 

  10. de Almeida, E.A., Di Mascio, P., Harumi, T., Warren Spence, D., Moscovitch, A., Hardeland, R., Cardinali, D.P., Brown, G.M., and Pandi-Perumal, S.R., Childs Nerv. Syst., 2011, vol. 27, p. 879.

    Article  PubMed  Google Scholar 

  11. Andreeva, N.I., Asnina, V.V., and Liberman, S.S., Pharm. Chem. J., 1999, p. 49.

    Google Scholar 

  12. Tanaka, D., Furusawa, K., Kameyama, K., Okamoto, H., and Doi, M., Neuropharmacology, 2007, vol. 53, p. 157.

    Article  CAS  PubMed  Google Scholar 

  13. Schickler, M., Knobler, H., Avraham, K.B., Elroy-Stein, O., and Groner, Y., EMBO J., 1989, vol. 8, p. 1385.

    Article  CAS  Google Scholar 

  14. Shah, A.J., de Biasi, V., Taylor, S.G., Roberts, C., Hemmati, P., Munton, R., West, A., Routledge, C., and Camilleri, P., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 1999, vol. 735, p. 133.

    Article  CAS  Google Scholar 

  15. Platt, S.R., Vet. J., 2007, vol. 173, p. 278.

    Article  CAS  PubMed  Google Scholar 

  16. Zhang, M.Y. and Beyer, C.E., J. Pharm. Biomed. Anal., 2006, vol. 40, p. 492.

    Article  CAS  PubMed  Google Scholar 

  17. Orlowska-Madjak, M., Acta Neurobiol. Exp., 2004, vol. 64, p. 177.

    Google Scholar 

  18. Chefer, V.I., Thompson, A.C., Zapata, A., and Shippenberg, T.S., Curr. Protocols Neurosci., 2009, vol. 47, p. 1.

    Article  Google Scholar 

  19. Santos-Fandila, A., Zafra-Gomez, A., Barronco, A., Navalon, A., Rueda, R., and Ramirez, M., Talanta, 2013, vol. 114, p. 79.

    Article  CAS  PubMed  Google Scholar 

  20. The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology, Geneva, 1994.

  21. The Fitness for Purpose of Analytical Methods, Eurachem Guide, 1998.

  22. ISO (The International Organization for Standardization)/IEC (The International Electrotechnical Commission) 17025: General Requirements for the Competence of Testing and Calibration Laboratories, 2005.

  23. The National Association of Testing Authorities, Australia (NATA) Technical Note 17: Guidelines for the Validation and Verification of Chemical Test Methods, 2009.

  24. Yenisoy-Karakaş, S., Gaga, E.O., Cankur, O., and Karakaş, D., Talanta, 2009, vol. 79, p. 1298.

    Article  CAS  PubMed  Google Scholar 

  25. Yenisoy-Karakaş, S., Food Chem., 2012, vol. 132, p. 1555.

    Article  CAS  PubMed  Google Scholar 

  26. Yenisoy-Karakaş, S., Anal. Chim. Acta, 2006, vol. 571, p. 298.

    Article  CAS  PubMed  Google Scholar 

  27. Semeraro, A., Altieri, I., Patriarca, M., and Menditto, A., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2009, vol. 877, p. 1209.

    Article  CAS  Google Scholar 

  28. Lecomte, F., Hubert, C., Demarche, S., De Bleye, C., Dispas, A., Jost, M., Frankenne, F., Ceccato, A., Rozet, E., and Hubert, Ph., J. Pharm. Biomed. Anal., 2012, vol. 57, p. 153.

    Article  CAS  PubMed  Google Scholar 

  29. Clarke, G., O’Mahony, S., Malone, G., and Dinan, T.G., J. Neurosci. Methods, 2007, vol. 160, p. 223.

    Article  CAS  PubMed  Google Scholar 

  30. de Montigny, P., Stobaugh, J.F., Givens, R.S., Carlson, R.G., Srinivasachar, K., Sternson, L.A., and Higuchi, T., Anal. Chem., 1987, vol. 59, p. 1096.

    Article  Google Scholar 

  31. Nussbaum, M.A., Przedwiecki, J.E., and Staerk, D.U., Anal. Chem., 1992, vol. 64, p. 1259.

    Article  CAS  PubMed  Google Scholar 

  32. Rizzo, V., Porta, C., Moroni, M., Scoglio, E., and Moratti, R., J. Chromatogr. B: Anal. Technol. Biomed. Life Sci., 2002, vol. 774, p. 17.

    Article  CAS  Google Scholar 

  33. Drijfhout, W.J., Grol, C.J., and Westerink, B.H.C., Eur. J. Pharmacol., 1996, vol. 308, p. 117.

    Article  CAS  PubMed  Google Scholar 

  34. Moujir, F., Santana, C., Hernandez, F.J., and Reiter, R.J., and Abreu, P., Neurosci. Lett., 1997, vol. 223, no. 2, p. 77.

    Article  CAS  PubMed  Google Scholar 

  35. Frese, T., Bach, A.G., Mühlbauer, E., Ponicke, K., Brömme, H.-J., Welp, A., and Peschke, E., Life Sci., 2009, vol. 85, p. 526.

    Article  CAS  PubMed  Google Scholar 

  36. Eurachem/CITAC Guide CG4: Quantifying Uncertainty in Analytical Measurement, 2012, 3rd ed.

  37. IUPAC Technical Report, Harmonised guidelines for the use of recovery information in analytical measurement, Pure Appl. Chem., 1999, vol. 71, p. 337.

    Google Scholar 

  38. Barwick, V.J. and Ellison, S.L.R., VAM Project 3.2.1 Development and Harmonization of Measurement Uncertainty Principles Part (d): Protocol for Uncertainty Evaluation from Validation Data, Report no. LGC/VAM/1998/088, 2000.

    Google Scholar 

  39. Pecorelli, I., Bibi, R., Fioroni, L., Piersanti, A., and Galarini, R., Anal. Chim. Acta, 2005, vol. 529, p. 15.

    Article  CAS  Google Scholar 

  40. Aslan-Sungur, G., Gaga, E.O., and Yenisoy-Karakaş, S., J. Chromatogr. A, 2015, vol. 1325, p. 40.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Abant İzzet Baysal University, Faculty of Science and Art, Department of Chemistry, Gölköy, Bolu, 14030, Turkey

    Özge Yetgin Çetin, Hatice Karadeniz & Serpil Yenisoy-Karakaş

  2. Abant İzzet Baysal University, Faculty of Science and Art, Department of Biology, Gölköy, Bolu, 14030, Turkey

    Alper Karakaş

Authors
  1. Özge Yetgin Çetin
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  2. Hatice Karadeniz
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  3. Alper Karakaş
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  4. Serpil Yenisoy-Karakaş
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Correspondence to Serpil Yenisoy-Karakaş.

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Yetgin Çetin, Ö., Karadeniz, H., Karakaş, A. et al. Determination of Melatonin Hormone and Neurotransmitters in Cerebrospinal Fluid: Method Validation and Uncertainty Calculations. J Anal Chem 73, 613–625 (2018). https://doi.org/10.1134/S1061934818060114

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  • DOI: https://doi.org/10.1134/S1061934818060114

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