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Development of PCR-based CNP marker of rice Waxy gene with confronting two-pair primers

  • Plant Genetics
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Abstract

Rice amylose content (AC) is a key determinant for grain end-use quality attributes. The base substitution ((G → T) at +1 loci of the first intron (In1) of Waxy (Wx), a major gene controlling AC in rice, results in decreased AC. A new SNP typing method of Wx In1 based on polymerase chain reaction with confronting two-pair primers (PCR-CTPP) was reported here: first its practicability was confirmed by 23 varieties with known SNP and AC; and then the segregation ratio at target SNP loci were checked and it fitted well for 1: 2: 1 single gene segregation; at last SNP typing and AC assay with 150 mini core collections (MCC) in China showed that average AC of 53 G type varieties (22.5%) was significantly higher than that of 97 T type varieties (13.7%) (p < 0.01) and the target SNP loci explained 77.8% AC variation. So this method could be used to estimate AC of rice variety roughly or in marker-assisted breeding, that is, using variety with known and desired AC as Wx allele donor parent and aided with crossbreed, backcross and marker-assisted selection (MAS) reported here, rice breeders could improve AC of varieties with comprehensively excellent performance to meet special end-products.

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Authors and Affiliations

  1. Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China

    H. Cai, D. Xu, L. Zhou, J. Cheng, J. Wu & A. You

  2. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China

    Z. Zhang

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  1. H. Cai
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  2. D. Xu
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  3. L. Zhou
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  4. J. Cheng
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  5. Z. Zhang
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  6. J. Wu
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  7. A. You
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Correspondence to H. Cai.

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Cai, H., Xu, D., Zhou, L. et al. Development of PCR-based CNP marker of rice Waxy gene with confronting two-pair primers. Russ J Genet 51, 673–676 (2015). https://doi.org/10.1134/S1022795415060034

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  • DOI: https://doi.org/10.1134/S1022795415060034

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