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A comparative genetic and cytogenetic mapping of wheat chromosome 5B using introgression lines

  • Plant Genetics
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Abstract

The genetic map of chromosome 5B has been constructed by using microsatellite (SSR) analysis of 381 plants from the F2 population produced by cross of the Chinese Spring (CS) and Renan cultivars. Initially, 180 SSR markers for the common wheat 5B chromosome have been used for analysis of these cultivars. The 32 markers able to detect polymorphism between these cultivars have been located on the genetic map of chromosome 5B. Cytogenetic mapping has involved a set of CS 5B chromosome deletion lines. Totally, 51 SSR markers have been located in ten regions (deletion bins) of this chromosome by SSR analysis of these deletion lines. Five genes—TaCBFIIIc-B10, Vrn-B1, Chi-B1, Skr, and Ph1—have been integrated into the cytogenetic map of chromosome 5B using the markers either specific of or tightly linked to the genes in question. Comparison of the genetic and cytogenetic maps suggests that recombination is suppressed in the pericentromeric region of chromosome 5B, especially in the short arm segment. The 18 markers localized to deletion bins 5BL16-0.79-1.00 and 5BL18-0.66-0.79 have been used to analyze common wheat introgression lines L842, L5366-180, L73/00i, and L21-4, carrying fragments of alien genomes in the terminal region of 5B long arm. L5366-180 and L842 lines carry a fragment of the Triticum timopheevii 5GL chromosome, while L73/00i and L21-4 lines, a fragment of the Aegilops speltoides 5SL chromosome. As has been shown, the translocated fragments in these four lines are of different lengths, allowing bin 5BL18-0.66-0.79 to be divided into three shorter regions. The utility of wheat introgression lines carrying alien translocations for increasing the resolution of cytogenetic mapping is discussed.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    E. M. Timonova, O. B. Dobrovol’skaya, E. M. Sergeeva, L. L. Bildanova & E. A. Salina

  2. UMR INRA-UBP Amélioration et Santé des Plantes, Clermont-Ferrand, 63100, France

    P. Sourdille & C. Feuillet

Authors
  1. E. M. Timonova
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  2. O. B. Dobrovol’skaya
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  3. E. M. Sergeeva
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  4. L. L. Bildanova
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  5. P. Sourdille
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  6. C. Feuillet
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  7. E. A. Salina
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Correspondence to E. A. Salina.

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Original Russian Text © E.M. Timonova, O.B. Dobrovol’skaya, E.M. Sergeeva, L.L. Bildanova, P. Sourdille, C. Feuillet, E.A. Salina, 2013, published in Genetika, 2013, Vol. 49, No. 12, pp. 1376–1384.

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Timonova, E.M., Dobrovol’skaya, O.B., Sergeeva, E.M. et al. A comparative genetic and cytogenetic mapping of wheat chromosome 5B using introgression lines. Russ J Genet 49, 1200–1206 (2013). https://doi.org/10.1134/S1022795413120132

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  • DOI: https://doi.org/10.1134/S1022795413120132

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