Abstract
The stability of inheritance of several genes in the Russian commercial strain Bifidobacterium lon- gum subsp. longum B379M during cultivation and maintenance under laboratory conditions has been studied. The examined genes code for probiotic characteristics, such as utilization of several sugars (lacA2 gene, encoding β-galactosidase; ara gene, encoding arabinosidase; and galA gene, encoding arabinogalactan endo-β-galactosidase); synthesis of bacteriocins (lans gene, encoding lanthionine synthetase); and mobile gene tet(W) of resistance to the antibiotic tetracycline. The other gene families studied include the genes responsible for signal transduction and adaptation to stress conditions in the majority of bacteria (serine/threonine protein kinases and the toxin-antitoxin systems of MazEF and RelBE families) and transcription regulators (genes encoding WhiB family proteins). Genomic DNA was analyzed by PCR using specially selected primers. A loss of the genes galA and tet(W) has been shown. It is proposed to expand the requirements on probiotic strains, namely, to control retention of the key probiotic genes using molecular biological methods.
Similar content being viewed by others
References
Shestakov, S.V., Metagenomics of Human Microbiom, “Usp. Sovrem. Biol., 2010, vol. 130, no. 6, pp. 531–543.
Stanton, C., Ross, R.P., Fitzgerald, G.F., and Van Sinderen, D., Fermented Functional Foods Based on Probiotics and Their Biogenic Metabolites, Curr. Opin. Biotech., 2005, vol. 16, pp. 198–203.
Huis, I.T., Veld, J.H., and Shortt, C., Selection Criteria for Probiotic Microorganisms, vol. 219 of International Congress and Symposium Series, London: Royal Society of Medicine Press, 1996.
Kosin, B. and Rakshit, S.K., Criteria for Production of Probiotics, Food Technol. Biotechnol., 2006, vol. 44, no. 3, pp. 371–379.
Van de Guchte, M., Serror, P., Chervaux, C., et al., Stress Responses in Lactic Acid Bacteria, Antonie van Leeuwenhoek, 2002, vol. 82, pp. 187–216.
Vaughan, E.E., Heilig, H.G., Ben-Amor, K., and de Vos, W.M., Diversity, Vitality and Activities of Intestinal Lactic Acid Bacteria and Bifidobacteria Assessed by Molecular Approaches, FEMS Microbiol. Rev., 2005, vol. 29, pp. 477–490.
Briczinski, E.P., Loquasto, J.R., Barrangou, R., et al., Strain-Specific Genotyping of Bifidobacterium animalis subsp. lactis by Using Single-Nucleotide Polymorphisms, Insertions, and Deletions, Appl. Environ. Microbiol., 2009, vol. 75, no. 23, pp. 7501–7508.
Regnault, B., Grimont, F., and Grimont, P.A.D., Universal Ribotyping Method Using a Chemically Labeled Oligonucleotide Probe Mixture, Res. Microbiol., 1997, vol. 148, no. 8, pp. 649–659.
Matsuki, T., Watanabe, K., and Fujimoto, J., Quantitative PCR with 16S rRNA-Gene-Targeted Species-Specific Primers for Analysis of Human Intestinal Bifidobacteria, Appl. Environ. Microbiol., 2004, vol. 70, no. 1, pp. 167–173.
Klijn, A., Mercenier, A., and Arigoni, F., Lessons from the Genomes of Bifidobacteria, FEMS Microbiol. Rev., 2005, vol. 29, pp. 491–509.
Hinz, S.W.A., Pastink, M.I., van den Broek, L.A.M., et al. Bifidobacterium longum Endogalactanase Liberates Galactotriose from Type I Galactans, Appl. Environ. Microbiol., 2005, vol. 71, no. 9, pp. 5501–5510.
Lee, J-H., Karamychev, V.N., Kozyavkin, S.A., et al., Comparative Genomic Analysis of the Gut Bacterium Bifidobacterium longum Reveals Loci Susceptible to Deletion during Pure Culture Growth, BMC Genomics, 2008, vol. 9, no. 247, pp. 1–16.
Gao, B., Paramanathan, R., Radhey, S.G., Signature Proteins That Are Distinctive Characteristics of Actinobacteria and Their Subgroups, Antonie van Leeuwenhoek, 2006, vol. 90, pp. 69–91.
Geiman, D.E., Raghunand, T.R., Agarwal, N., and Bishai, W.R., Differential Gene Expression in Response to Exposure to Antimycobacterial Agents and Other Stress Conditions among Seven Mycobacterium tuberculosis whiB-Like Genes, Antimicrob. Agents Chemother., 2006, vol. 50, pp. 2836–2841.
Averina, O.V., Zakharevich, N.V., and Danilenko, V.N., Identification and Characterization of WhiB-Like Proteins of the Bifidobacteria Genus, Anaerobe, 2012, vol. 5, no. 4, pp. 421–429.
Bifidobacteria: Genomics and Molecular Aspects, Mayo, B. and Sinderen, D.V., Eds., 2010.
Moubareck, C., Gavini, F., Vaugien, L., et al., Antimicrobial Susceptibility of Bifidobacteria, J. Antimicrob. Chemother., 2005, vol. 55, pp. 38–44.
Masco, L., Van Hoorde, K., De Brandt, E., et al., Antimicrobial Susceptibility of Bifidobacterium Strains from Humans, Animals and Probiotic Products, J. Antimicrob. Chemother., 2006, vol. 58, pp. 85–94.
Ammor, M.S., Florez, A.B., Alvarez-Martin, P., et al., Analysis of Tetracycline Resistance tet(W) Genes and Their Flanking Sequences in Intestinal Bifidobacterium Species, J. Antimicrob. Chemother., 2008, vol. 62, no. 4, pp. 688–693.
Zakharevich, N.V., Osolodkin, D.I., Artamonova, I.I., et al., Signatures of the ATP Binding Pocket as a Basis for Structural Classification of the Serine/Threonine Protein Kinases of Gram-Positive Bacteria, Proteins: Struct., Funct. Bioinf., in press (DOI10.1002/prot.24032).
Danilenko, V.N., Osolodkin, D.I., Lakatosh, S.A., and Preobrazhenskaya, M.N., Bacterial Eukaryotic Type Serine-Threonine Protein Kinases: From Structural Biology to Targeted Anti-Infective Drug Design, Curr. Topics Medic. Chem., 2011, vol. 11, pp. 1352–1369.
Nezametdinova, V.Z., Alekseeva, M.G., Mironcheva, T.A., and Danilenko, V.N., Structural and Functional Characterization of Eukaryotic Type Serine-Threonine Protein Kinases in the Bifidobacterium longum B379M Strain, Abstracts of 2nd International Congress—Partnering and Exhibition on Biotechnology and Bioenergy, Vasilov, R.G., Ed., Moscow: Copyring, 2010.
Prozorov, A.A. and Danilenko, V.N., Toxin-Antitoxin Systems in Bacteria: Apoptotic Tools or Metabolic Regulators?, Mikrobiologiya, 2010, vol. 79, no. 2, pp. 147–159.
Nogueira, T., Rankin, D.J., Touchon, M., et al., Horizontal Gene Transfer of the Secretome Drives the Evolution of Bacterial Cooperation and Virulence, Curr. Biol., 2009, vol. 19–20, p. 1683.
FAO/WHO: Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food, 2002.
Arumugam, M., Raes, J., Pelletier, E., et al., Enterotypes of the Human Gut Microbiome, Nature, 2011, vol. 473, no. 12, pp. 174–180.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.V. Averina, V.Z. Nezametdinova, M.G. Alekseeva, V.N. Danilenko, 2012, published in Genetika, 2012, Vol. 48, No. 11, pp. 1287–1296.
Rights and permissions
About this article
Cite this article
Averina, O.V., Nezametdinova, V.Z., Alekseeva, M.G. et al. Genetic instability of probiotic characteristics in the Bifidobacterium longum subsp. longum B379M strain during cultivation and maintenance. Russ J Genet 48, 1103–1111 (2012). https://doi.org/10.1134/S1022795412110026
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1022795412110026