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Effect of mutations in Drosophila melanogaster tumor suppressor Merlin on proliferation and differentiation of wing cells

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Abstract

Experiments on transplantation of wing imaginal discs homozygous for a mutation in the tumor suppressor gene Merlin have demonstrated that this mutation does not induce malignant tumors. Marking of the wing disc compartment borders by specific antibodies showed the absence of essential compartment border defects in case of the Merlin mutation. Drosophila melanogaster cells mutant for Merlin have shorter cell cycle than normal cells. Proliferation of imaginal discs lasts longer in case of the mutation. It is known that beginning from some moment of development, wing veins serve as clonal restriction lines that cannot be crossed by growing mosaic clones. We showed that the Merlin mutation leads to depression of vein clonal restriction property. This means that this gene is involved not only in the control of cell proliferation, but also in the control of cell mobility and adhesion.

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Authors and Affiliations

  1. Institute of Chemical Biology and Fundamental Medicine, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    T. D. Dubatolova, S. A. Kopyl & L. V. Omelyanchuk

  2. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    N. A. Bulgakova & N. V. Dorogova

  3. Department of Genetics, Novosibirsk State University, Novosibirsk, 630090, Russia

    L. V. Omelyanchuk

  4. Center for Childhood Cancer, Children’s Research Institute, Department of Pediatrics, Ohio State University College of Medicine, Columbus, OH, 43205, USA

    L.-Sh. Chang

Authors
  1. T. D. Dubatolova
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  2. S. A. Kopyl
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  3. N. A. Bulgakova
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  4. N. V. Dorogova
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  5. L. V. Omelyanchuk
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  6. L.-Sh. Chang
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Corresponding author

Correspondence to L. V. Omelyanchuk.

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Original Russian Text © T.D. Dubatolova, S.A. Kopyl, N.A. Bulgakova, N.V. Dorogova, L.V. Omelyanchuk, L.-Sh. Chang, 2010, published in Genetika, 2010, Vol. 46, No. 2, pp. 187–193.

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Dubatolova, T.D., Kopyl, S.A., Bulgakova, N.A. et al. Effect of mutations in Drosophila melanogaster tumor suppressor Merlin on proliferation and differentiation of wing cells. Russ J Genet 46, 164–169 (2010). https://doi.org/10.1134/S1022795410020055

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  • DOI: https://doi.org/10.1134/S1022795410020055

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