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Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences

  • Plant Genetics
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Abstract

In this study, the relationships of five varieties in Perilla frutescens (L.) Britton were analyzed. All the varieties showed the same chromosome number 2n = 40, and the banding patterns of PI-DAPI staining and the FISH results of 45S rDNA probe were on the same site of the chromosome pair. The 5S rRNA gene spacers were ranged from 413 bp in P. frutescens var. purpurascens and 408 bp in var. frutescens. The varieties analyzed here were clustered into two clades according to the phylogenetic analysis. The sequence identity of 96% between var. auriculato-dentata and var. arguta suggested they could be considered the same variety.

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Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University, Wuhan, 430072, China

    Y. Diao, Y. Miao & Z. Hu

  2. College of Life Science and Technology, Chongqing University of Arts and Sciences, Yongchuan, 402168, China

    Y. Diao

  3. Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi, 445000, China

    X. Lin & C. Liao

  4. Perilla Research Institute, Yunnan Agricultural University, Kunming, 650201, China

    F. Guo

Authors
  1. Y. Diao
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  2. Y. Miao
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  3. X. Lin
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  4. C. Liao
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  5. F. Guo
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  6. Z. Hu
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Correspondence to F. Guo.

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Diao, Y., Miao, Y., Lin, X. et al. Comparative analysis of five varieties in Perilla frutescens (L.) Britton by 45S rDNA FISH and 5S rDNA sequences. Russ J Genet 45, 440–444 (2009). https://doi.org/10.1134/S1022795409040085

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  • DOI: https://doi.org/10.1134/S1022795409040085

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