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Search for destruction factors of bacterial biofilms: Comparison of phage properties in a group of Pseudomonas putida bacteriophages and specificity of their halo-formation products

  • Genetics of Microorganisms
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Abstract

Comparison of Pseudomonas putida group of phages attributed to five species (af, ϕ15, ϕ27, ϕ2F, and pf16) with their common property of halo-formation (formation of lightening zones) around phage plaques was conducted. The halo around phage plaques appears as a result of reduction or disappearance of bacterial polysaccharide capsules. The concentration of viable bacteria remains unchanged within the halo. A comparison of specificities of halo-formation products from various phages was conducted by a simple method. These products were shown to be highly specific and inactive on other species of pseudomonads. Phage-resistant P. putida mutants scored with respect to various phages, which lost phage adsorption ability, were tolerant to the effect of halo-formation products in most cases. Apparently, the capsular polysaccharides, which serve as a substrate for depolymerases and are the primary phage receptors, may be often lost. Results of partial sequencing of the af phage genome revealed an open reading frame that encodes the enzyme transglycosylase similar rather to transglycosylases of oligotrophic bacteria belonging to different species than to lysozymes of other phages. Possibly, it is a polyfunctional enzyme combining functions of lysozyme and an enzyme that executes the penetration of phage particle across extracellular slime and capsule.

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia

    O. V. Shaburova, S. V. Krylov, V. P. Veiko, E. A. Pleteneva, M. V. Burkal’tseva & V. N. Krylov

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 119997, Russia

    K. A. Miroshnikov, N. N. Sykilinda, V. A. Kadykov & V. V. Mesyanzhinov

  3. Division of Gene Technology, Katholieke Universiteit Leuven, B-3001, Leuven, Belgium

    A. Cornelissen, R. Lavigne & G. Volckaert

Authors
  1. O. V. Shaburova
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  2. S. V. Krylov
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  3. V. P. Veiko
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  4. E. A. Pleteneva
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  6. K. A. Miroshnikov
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  7. A. Cornelissen
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  8. R. Lavigne
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  11. V. V. Mesyanzhinov
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  13. V. N. Krylov
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Correspondence to S. V. Krylov.

Additional information

Original Russian Text © O.V. Shaburova, S.V. Krylov, V.P. Veiko, E.A. Pleteneva, M.V. Burkal’tseva, K.A. Miroshnikov, A. Cornelissen, R. Lavigne, N.N. Sykilinda, V.A. Kadykov, V.V. Mesyanzhinov, G. Volckaert, V.N. Krylov, 2009, published in Genetika, 2009, Vol. 45, No. 2, pp. 185–195.

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Shaburova, O.V., Krylov, S.V., Veiko, V.P. et al. Search for destruction factors of bacterial biofilms: Comparison of phage properties in a group of Pseudomonas putida bacteriophages and specificity of their halo-formation products. Russ J Genet 45, 161–170 (2009). https://doi.org/10.1134/S1022795409020057

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  • DOI: https://doi.org/10.1134/S1022795409020057

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