Abstract
Using a model system in which the expression of the reporter gene lacZ is under the control of five deleted variants of the copia retrotransposon regulatory region, which includes the 5′-long terminal repeat (LTR) and the 5′-untranslated region (5′-UTR), their contribution to the control of retrotransposon activity in different organs of males and females of Drosophila melanogaster was analyzed. The whole regulatory region provides expression of the reporter gene at the embryonic stage, and in larvae and adult flies only in generative organs. The 5′-end of LTR harbors a positive regulator that determines expression of the retrotransposon in organs of all types. The 3′-end of LTR harbors a negative regulator, which is sex- and time-specific: it represses copia expression in generative organs of males at all stages of development, and only at the imaginal stage in somatic tissues, without any effect on the expression of the retrotransposon in females. 5′-UTR contains a negative regulator of copia expression: it decreases the expression in embryos and generative organs and blocks it in somatic tissues. It may be suggested that a complex set of regulatory elements was formed in the course of the evolution of the retrotransposon, which made it possible to maintain a certain level of its expression in different types of cells and tissues and at different stages of development and, thus, to limit the harm caused to the host and provide the possibility for the retrotransposon to exist in the host genome over many generations.
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Pelisson, A., Song, S.U., Prud’homme, N., et al., Gypsy Transposition Correlates with the Production of a Retroviral Envelope-Like Protein under the Tissue-Specific Control of the Drosophila flamenco Gene, EMBO J., 1994, vol. 13, pp. 4401–4411.
Busseau, I., Chaboissier, M.C., Pelisson, A., and Bucheton, A., I Factors in Drosophila melanogaster: Transposition under Control, Genetica, 1994, vol. 93, pp. 101–116.
Pasyukova, E.G., Nuzhdin, S.V., Li, W., and Flavell, A.J., Germ Line Transposition of the copia Retrotransposon in Drosophila melanogaster Is Restricted to Males by Tissue-Specific Control of copia RNA Levels, Mol. Gen. Genet., 1997, vol. 255, pp. 115–124.
Pasyukova, E.G., Nuzhdin, S.V., and Filatov, D.A., The Relationship between the Rate of Transposition and Transposable Element Copy Number for copia and Doc Retrotransposons of Drosophila melanogaster, Genet. Res., 1998, vol. 72, pp. 1–11.
Borie, N., Maisonhaute, C., Sarrazin, C., et al., Tissue-Specificity of 412 Retrotransposon Expression in Drosophila simulans and Drozophila melanogaster, Heredity, 2002, vol. 89, pp. 247–252.
Tcheressiz, S., Calco, V., Arnaud, F., et al., Expression of the Idefix Retrotransposon in Early Follicle Cells in the Germarium of Drosophila melanogaster Is Determined by Its LTR Sequences and a Specific Genomic Context, Mol. Genet. Genomics, 2002, vol. 267, pp. 133–141.
Arkhipova, I.R. and Ilyin, Y.V., Control of Transcription of Drosophila Retrotransposons, BioEssays, 1992, vol. 14, pp. 161–168.
Yun, Y. and Davis, R., Copia RNA Levels Are Elevated in dunce Mutants and Modulated by CAMP, Nucleic Acids Res., 1989, vol. 17, pp. 8313–8326.
Cavarec, L. and Heidmann, T., The Drosophila copia Retrotransposon Contains Binding Sites for Transcriptional Regulation by Homeoproteins, Nucleic Acids Res., 1993, vol. 21, pp. 5041–5049.
Sneddon, A. and Flavell, A.J., The Transcriptional Control Regions of the copia Retrotransposon, Nucleic Acids Res., 1989, vol. 17, pp. 4025–4035.
Wilson, S., Matyunina, L.V., and McDonald, J.F., An Enhancer Region within the copia Untranslated Leader Contains Binding Sites for Drosophila Regulatory Proteins, Gene, 1998, vol. 209, pp. 239–246.
Cavarec, L., Jensen, S., Casella, J., et al., Molecular Cloning and Characterization of a Transcription Factor for the copia Retrotransposon with Homology to the BTB-Containing Lola Neurogenic Factor, Mol. Cell. Biol., 1997, vol. 17, pp. 482–494.
Morozova, T.V., Tcybulko, E.A., Filatov, D.A., and Pasyukova, E.G., Regulatory Elements of copia Retrotransposon, Controlling the Level of Its Expression in Testes of Drosophila melanogaster, Russ. J. Genet., 2004, vol. 40, no. 2, pp. 173–179.
Ilyin, Y.V., Tchurikov, N.A., Ananiev, E.V., et al., Studies of the DNA Fragments of Mammals and Drosophila Containing Structural Genes and Adjacent Sequences, Cold Spring Harbor Symp. Quant. Biol., 1978, vol. 42, pp. 959–969.
Echalier, G., Drosophila Cells in Culture, New York: Academic, 1997.
Ding, D. and Lipshitz, H.D., Spatially Regulated Expression of Retrovirus-Like Transposons during D. melanogaster Embryogenesis, Genet. Res., 1994, vol. 64, pp. 167–181.
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Original Russian Text © T.V. Morozova, E.A. Tcybulko, E.G. Pasyukova, 2009, published in Genetika, 2009, Vol. 45, No. 2, pp. 169–177.
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Morozova, T.V., Tcybulko, E.A. & Pasyukova, E.G. Regularory elements of the copia retrotransposon determine different levels of expression in different organs of males and females of Drosophila melanogaster . Russ J Genet 45, 147–154 (2009). https://doi.org/10.1134/S1022795409020033
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DOI: https://doi.org/10.1134/S1022795409020033