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Interactive effects of salicylic acid and nitric oxide on soybean plants under NaCl salinity

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Abstract

The effects of salicylic acid (SA), sodium nitroprusside (SNP), a nitric oxide donor, and their combination (SA+SNP) on some physiological parameters of 23-day-old soybean seedlings grown under saline and nonsaline conditions were studied. The changes in the leaf area, shoot fresh and dry weights, contents of chlorophylls and carotenoids, amounts of MDA and hydrogen peroxide showed that the addition of 100 μM SA and/or 100 μM SNP markedly declined the oxidative damage to soybean plants induced by 50 and 100 μM NaCl. Our results proved that combined action of SA and nitric oxide donor significantly activated catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX), which contributed to the decay of H2O2 in soybean leaves under NaCl toxicity. The protective action of (SA+SNP) against saltinduced oxidative damage was often more efficient than effects of SA and SNP alone. We also observed that the accumulation of proline was apparently accelerated by these substances under salt stress. As well, it was observed that the interaction between SA and nitric oxide had synergistic effects in decreasing of the damages induced by NaCl salinity.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbic acid

CAT:

catalase

cGMP:

cyclic guanosine monophosphate

Chl:

chlorophyll

GPX:

guaiacol peroxidase

GR:

glutathione reductase

SA:

salicylic acid

SNP:

sodium nitroprusside

SOD:

superoxide dismutase

TBARS:

thiobarbituric acid-reactive substances

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    M. Simaei, R. A. Khavarinejad, S. Saadatmand & H. Fahimi

  2. Faculty of Biological Sciences, Shahid Beheshti G.C. University, Tehran, Iran

    F. Bernard

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  1. M. Simaei
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  2. R. A. Khavarinejad
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  3. S. Saadatmand
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  4. F. Bernard
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  5. H. Fahimi
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Correspondence to M. Simaei.

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This text was submitted by the autors in English.

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Simaei, M., Khavarinejad, R.A., Saadatmand, S. et al. Interactive effects of salicylic acid and nitric oxide on soybean plants under NaCl salinity. Russ J Plant Physiol 58, 783–790 (2011). https://doi.org/10.1134/S1021443711050220

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  • DOI: https://doi.org/10.1134/S1021443711050220

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