Abstract
Resveratrol is a phytoalexin with antibacterial, antiviral and cancer-preventing effects. The objective of the study was to identify PAL and STS genes of Vitis amurensis Rupr. encoding the phenylalanine ammonia-lyases (PAL) and stilbene synthases (STS), the key enzymes involved in the resveratrol biosynthesis. A V. amurensis Rupr. cell culture characterized by low resveratrol level was chosen as a model object. Salicylic acid (SA), a known secondary metabolism inducing agent, was used for enhancement of resveratrol production in this culture. PAL and STS gene expression was analyzed using the reverse transcription PCR and real-time PCR techniques. SA was originally found to specifically enhance the expression of VaPAL3, VaSTS2, VaSTS3, VaSTS4, VaSTS5, VaSTS6, and VaSTS8 of multigene families VaPAL and VaSTS. The results obtained were compared with the earlier published data on PAL and STS gene expression in the rolB transformed V. amurensis cell cultures characterized by high levels of resveratrol. The effects of SA treatment and the rolB transformation on VaPAL and VaSTS gene expression were found to be considerably different.
Similar content being viewed by others
Abbreviations
- BA:
-
benzyladenine
- DFR:
-
dihydroflavanol-4-reductases
- RT-PCR:
-
real-time quantitative PCR
- PAL:
-
phenylalanine ammonia-lyase
- SA:
-
salicylic acid
- STS:
-
stilbene synthase
References
Aggarwal, B.B., Bhardwaj, A., Aggarwal, R.S., Seeram, N.P., Shishodia, S., and Takada, Y., Role of Resveratrol in Prevention and Therapy of Cancer: Preclinical and Clinical Studies, Anticancer Res., 2004, vol. 24, pp. 2783–2840.
Docherty, J.J., Fu, M.M., Stiffler, B.S., Limperos, R.J., Pokabla, C.M., and DeLucia, A.L., Resveratrol Inhibition of Herpes Simplex Virus Replication, Antiviral. Res., 1999, vol. 43, pp. 145–155.
Daroch, F., Hoeneisen, M., Gonzalez, C.L., Kawaguchi, F., Salgado, F., Solar, H., and Garcia, A., In Vitro Antibacterial Activity of Chilean Red Wines against Helicobacter pylori, Microbios, 2001, vol. 104, pp. 79–85.
Pervaiz, S., Resveratrol: From Grapevines to Mammalian Biology, Faseb J., 2003, vol. 17, pp. 1975–1985.
Langcake, P. and Pryce, R.J., A New Class of Phytoalexins from Grapevines, Experientia, 1977, vol. 33, pp. 151–152.
Rupprich, N., Hildebrand, H., and Kindl, H., Substrate Specificity In Vivo and In Vitro in the Formation of Stilbenes — Biosynthesis of Rhaponticin, Arch. Biochem. Biophys., 1980, vol. 200, pp. 72–78.
Jaillon, O., Aury, J.M., Noel, B., Policriti, A., Clepet, C., Adam-Blondon, A.F., Weissenbach, J., Quetier, F., and Wincker, P., The Grapevine Genome Sequence Suggests Ancestral Hexaploidization in Major Angiosperm Phyla, Nature, 2007, vol. 449, p. 463.
Velasco, R., Zharkikh, A., Troggio, M., Cartwright, D.A., Cestaro, A., Pruss, D., Pindo, M., Fitzgerald, L.M., Vezzulli, S., Reid, J., Malacarne, G., Iliev, D., Coppola, G., Wardell, B., Micheletti, D., Macalma, T., Facci, M., Mitchell, J.T., Perazzolli, M., Eldredge, G., Gatto, P., Oyzerski, R., Moretto, M., Gutin, N., Stefanini, M., Chen, Y., Segala, C., Davenport, C., Dematté, L., Mraz, A., Battilana, J., Stormo, K., Costa, F., Tao, Q., Si-Ammour, A., Harkins, T., Lackey, A., Perbost, C., Taillon, B., Stella, A., Solovyev, V., Fawcetté, J.A., Sterck, L., Vandepoele, K., Grando, S.M., Toppo, S., Moser, C., Lanchbury, J., Bogden, R., Skolnick, M, Sgaramella, V., Bhatnagar, S.K., Fontana, P., Gutin, A., van de Peer, Y., Salamini, F., and Viola, R., A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety, PLoS One, 2007, vol. 2, p. e1326.
Sparvoli, F., Martin, C., Scienza, A., Gavazzi, G., and Tonelli, C., Cloning and Molecular Analysis of Structural Genes Involved in Flavonoid and Stilbene Biosynthesis in Grape (Vitis vinifera L.), Plant Mol. Biol., 1994, vol. 24, pp. 743—755.
Kao, Y.Y., Harding, S.A., and Tsai, C.J., Differential Expression of Two Distinct Phenylalanine Ammonia-Lyase Genes in Condensed Tannin-Accumulating and Lignifying Cells of Quaking Aspen, Plant Physiol., 2002, vol. 130, pp. 796–807.
Chaman, M.E., Copaja, S.V., and Argandoña, V.H., Relationships between Salicylic Acid Content, Phenylalanine Ammonia-Lyase (PAL) Activity, and Resistance of Barley to Aphid Infestation, J. Agric. Food Chem., 2003, vol. 51, pp. 2227–2231.
Raes, J., Rohde, A., Christensen, J.H., van de Peer, Y., and Boerjan, W., Genome-Wide Characterization of the Lignification Toolbox in Arabidopsis, Plant Physiol., 2003, vol. 133, pp. 1051–1071.
Cochrane, F.C., Davin, L.B., and Lewis, N.G., The Arabidopsis Phenylalanine Ammonia-Lyase Gene Family: Kinetic Characterization of the Four PAL Isoforms, Phytochemistry, 2004, vol. 65, pp. 1557–1564.
Kiselev, K.V., Dubrovina, A.S., and Bulgakov, V.P., Phenylalanine Ammonia-Lyase and Stilbene Synthase Gene Expression in rolB Transgenic Cell Cultures of Vitis amurensis, Appl. Microbiol. Biotechnol., 2009, vol. 82, pp. 647–655.
Kiselev, K.V., Dubrovina, A.S., Veselova, M.V., Bulgakov, V.P., Fedoreyev, S.A., and Zhuravlev, Y.N., The rolB Gene-Induced Overproduction of Resveratrol in Vitis amurensis Transformed Cells, J. Biotechnol., 2007, vol. 128, pp. 681–692.
Estruch, J., Schell, J., and Spena, A., The Protein Encoded by the rolB Plant Oncogene Hydrolyses Indole Glucosides, EMBO J., 1991, vol. 10, pp. 3125–3128.
Nilsson, O., Crozier, A., Schmülling, T., Sandberg, G., and Olsson, O., Indole-3-Acetic Acid Homeostasis in Transgenic Tobacco Plants Expressing the Agrobacterium rhizogenes rolB Gene, Plant J., 1993, vol. 3, pp. 681–689.
Filippini, F., Rossi, V., Marin, O., Trovato, M., Costantino, P., Downey, P.M., Lo, Schiavo, F., and Terzi, M., A Plant Oncogene as a Phosphatase, Nature, 1996, vol. 379, pp. 499–500.
Moriuchi, H., Okamoto, C., Nishihama, R., Yamashita, I., Machida, Y., and Tanaka, N., Nuclear Localization and Interaction of RolB with Plant 14-3-3 Proteins Correlates with Induction of Adventitious Roots by the Oncogene rolB, Plant J., 2004, vol. 38, pp. 260–275.
Bekesiova, I., Nap, J.P., and Mlynarova, L., Isolation of High Quality DNA and RNA from Leaves of the Carnivorous Plant Drosera rotundifolia, Plant Mol. Biol. Rep., 1999, vol. 17, pp. 269–277.
Kiselev, K.V., Kusaykin, M.I., Dubrovina, A.S., Bezverbny, D.A., Zvyagintseva, T.N., and Bulgakov, V.P., The rolC Gene Induces Expression of a Pathogenesis-Related β-1,3-Glucanase in Transformed Ginseng Cells, Phytochemistry, 2006, vol. 67, pp. 2225–2231.
Persiyanova, E.V., Kiselev, K.V., Bulgakov, V.P., Timchenko, N.F., Chernoded, G.K., and Zhuravlev, Yu.N., Defense Response Mechanisms of Ginseng Callus Cultures Induced by Yersinia pseudotuberculosis, a Human Pathogen, Russ. J. Plant Physiol., 2008, vol. 55, pp. 748–755.
Dubrovina, A.S., Kiselev, K.V., Veselova, M.V., Isaeva, G.A., Fedoreyev, S.A., and Zhuravlev, Y.N., Enhanced Resveratrol Accumulation in rolB Transgenic Cultures of Vitis amurensis Correlates with Unusual Changes in CDPK Gene Expression, J. Plant Physiol., 2009, vol. 166, pp. 1194–1206.
Kiselev, K.V. and Chernoded, G.K., Somatic Embryogenesis in the Panax ginseng Cell Culture Induced by the rolC Oncogene Is Associated with Increased Expression of WUS and SERK Genes, Russ. J. of Genetics, 2009, vol. 45, pp. 445–452.
Des Marais, D.L. and Rausher, M.D., Escape from Adaptive Conflict after Duplication in an Anthocyanin Pathway Gene, Nature, 2008, vol. 454, pp. 762–U85.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © K.V. Kiselev, A.S. Dubrovina, G.A. Isaeva, Y.N. Zhuravlev, 2010, published in Fiziologiya Rastenii, 2010, Vol. 57, No.3, pp. 441–448.
Rights and permissions
About this article
Cite this article
Kiselev, K.V., Dubrovina, A.S., Isaeva, G.A. et al. The effect of salicylic acid on phenylalanine ammonia-lyase and stilbene synthase gene expression in Vitis amurensis Cell Culture. Russ J Plant Physiol 57, 415–421 (2010). https://doi.org/10.1134/S1021443710030143
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1021443710030143