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Development and validation of a new high-performance liquid chromatographic method for the loperamid hydrochloride determination in drugs

  • Biophysical Chemistry
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Abstract

A selective, precise and new high-performance liquid chromatographic method for the analysis of loperamid hydrochloride in pharmaceutical formulations was developed and validated. The mobile phase consisting buffer (sodium-octansulphonate, triethylamine and ammonium hydroxide) in water: acetonitriie (45: 55, v/v) (pH 3.2). The absorbance was monitored with a DAD detector at 226 nm. The flow rate was 1.5 cm3 min−1. The linearity (r = 0.9947) and the recovery (98.58–100.42%) were found to be satisfactory. The detection and quantitation limits were found to be 0.95 and 3.12 μg cm−3. The results demonstrated that the procedure was accurate, precise and reproducible. It can be suitably applied for the estimation of lopera-mid hydrochloride in pharmaceutical formulations.

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Authors and Affiliations

  1. Faculty of Technology, Bulevar Oslobođenja 124, RS-16000, Leskovac, Serbia

    G. S. Nikolić & I. Savić

  2. Pharmaceutical and Chemical Industry Zdravlje-Actavis, Vlajkova 199, RS-16000, Leskovac, Serbia

    V. Marinković

Authors
  1. G. S. Nikolić
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  2. I. Savić
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  3. V. Marinković
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Correspondence to G. S. Nikolić.

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Nikolić, G.S., Savić, I. & Marinković, V. Development and validation of a new high-performance liquid chromatographic method for the loperamid hydrochloride determination in drugs. Russ. J. Phys. Chem. 83, 1609–1611 (2009). https://doi.org/10.1134/S0036024409090349

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  • DOI: https://doi.org/10.1134/S0036024409090349

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