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Differential expression of microRNA-2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm

  • Molecular Biology of the Cell
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Abstract

MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate a gene expression at the post-transcriptional level. Using in silico screening, we found that the 3′-untranslated regions of the P25 gene mRNA are perfectly complementary to nucleotides 2–8 at the 5′ end of the miRNA-2b (miR-2b). The expression of miR-2b and the P25 gene in posterior silk gland of the fifth instar larval silkworm was investigated using real-time PCR detection method. The results indicated that expression of the P25 gene was very high in the posterior silk gland during the fifth instar larvae, whereas a level of miR-2b sharply decreased until reaching the lowest one on the 8th day. The expression patterns of miR-2b and P25 gene indicate that miR-2b might act as a fine-tuning regulator of expression of the P25 gene at the post-transcriptional level.

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Authors and Affiliations

  1. The Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Jiang Su University of Science and Technology, 212018, Jiangsu Province, PR China

    Yong Huang, Xing Jia Shen, Shun Ming Tang, Sheng Peng Wang & Qiao Ling Zhao

  2. School of Information Science and Technology of Xiamen University, 361005, Fujian Province, PR China

    Quan Zou

  3. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 212018, Jiangsu Province, PR China

    Yong Huang, Xing Jia Shen, Shun Ming Tang, Sheng Peng Wang & Qiao Ling Zhao

Authors
  1. Yong Huang
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  2. Quan Zou
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  3. Xing Jia Shen
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  4. Shun Ming Tang
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Correspondence to Xing Jia Shen.

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Huang, Y., Zou, Q., Shen, X.J. et al. Differential expression of microRNA-2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm. Mol Biol 45, 576–581 (2011). https://doi.org/10.1134/S0026893311040133

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  • DOI: https://doi.org/10.1134/S0026893311040133

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