Skip to main content
SpringerLink
Log in

Expression of FTL and FTH genes encoding ferritin subunits in lung and renal carcinomas

  • Genomics. Transcriptomics. Proteomics
  • Published:
Molecular Biology Aims and scope Submit manuscript

Abstract

Numerous human cancers are accompanied with the increase of ferritin content in blood serum. Ferritin is composed of light and heavy chains, encoded by FTL and FTH genes, respectively. The analysis of EST database showed that the expression of FTL and FTH genes in lung tumors is decreased compared to normal tissues, and is not altered in renal cancer cells. The alteration of mRNA corresponding to FTL and FTH genes was estimated by real-time PCR in primary lung and kidney tumors. A significant and frequent inhibition of FTL and FTH gene expression (on average by 11 and 9 times, in 83% (33/40) and 73% (11/15) of cases, respectively) was detected in primary squamous lung carcinoma. The expression of these genes was not altered so significantly (by 6 and 3 times, in 58% and 27% of samples) in clear cell renal cell carcinoma. Our work reports for the first time the down-regulation of FTL gene expression at the first stage of lung cancer (10/10), and proposes this gene as a potential oncomarker for early diagnosis. The FTL mRNA content may be quantified by non-competitive hybridization on expression DNA microarrays. The possible causes of a serum ferritin increase in lung squamous cell carcinoma and clear cell renal cell carcinoma are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Koorts A.M., Viljoen M. 2007. Ferritin and ferritin isoforms II: Protection against uncontrolled cellular proliferation, oxidative damage and inflammatory processes. Arch. Physiol. Biochem. 113(2), 55–64.

    Article  CAS  PubMed  Google Scholar 

  2. Koorts A.M., Viljoen M. 2007. Ferritin and ferritin isoforms I: Structure-function relationships, synthesis, degradation and secretion. Arch. Physiol. Biochem. 113(1), 30–54.

    Article  CAS  PubMed  Google Scholar 

  3. Zaleska M., Szturmowicz M., Zych J., Remiszewski P., Plodziszewskal M., Szopinski J., Zaleska J., Demkow U., Langfort R., Roszkowska-Sliz B., Roszkowski-Sliz K. 2005. The significance of the serum tumour markers as a prognostic and predictor factors in nonsmall cell lung cancer patients. Pneumonol. Alergol. Pol. 73(3), 270–276.

    PubMed  Google Scholar 

  4. Mertz J.R., Theil E.C. 1983. Subunit dimers in sheep spleen apoferritin: The effect on iron storage. J. Biol. Chem. 258(19), 11719–11726.

    CAS  PubMed  Google Scholar 

  5. Essen A., Ozen H., Ayhan A., Ergen A., Tasar C., Remzi F. 1991. Serum ferritin: A tumor marker for renal cell carcinoma. J. Urol. 145(6), 1134–1137.

    CAS  PubMed  Google Scholar 

  6. Grail A., Hancock B.W., Harrison P. 1092. Serum ferritin in normal individuals and in patients with malignant lymphoma and chronic renal failure measured with seven different commercial immunoassay techniques. J. Clin. Pathol. 35, 1204–1212.

    Article  Google Scholar 

  7. Milman N., Pedersen L. 2002. The serum ferritin concentration is a significant prognostic indicator of survival in primary lung cancer. Oncol. Rep. 9, 193–198.

    CAS  PubMed  Google Scholar 

  8. Kashyap M.K., Kumar A., Emelianenko N., Kashyap A., Kaushik R., Huang R., Khullar M., Sharma S.K., Singh S.K., Bhargave A.K., Upadhyaya S.K. 2005. Biochemical and molecular markers in renal cell carcinoma: An update and future prospects. Biomarkers. 10(4), 258–294.

    Article  CAS  PubMed  Google Scholar 

  9. Ozen H., Uygur C., Sahin A., Tekgul S., Ergen A., Remzi D. 1995. Clinical significance of serum ferritin in patients with renal cell carcinoma. Urology. 46(4), 494–498.

    Article  CAS  PubMed  Google Scholar 

  10. Miyata Y., Koga S., Nishikido M., Hayashi T., Kanetake H. 2001. Relationship between serum ferritin levels and tumour status in patients with renal cell carcinoma. BJU Int. 88(9), 974–977.

    Article  CAS  PubMed  Google Scholar 

  11. Fracchia A., Ubbiali A., El Bitar O., Pacetti M., Sommariva E., Arreghini M., Longhini E., Bonalumi G.P. 1999. A comparative study on ferritin concentration in serum and bilateral bronchoalveolar lavage fluid of patients with peripheral lung cancer versus control subjects. Oncology. 56(3), 181–188.

    Article  CAS  PubMed  Google Scholar 

  12. Shen C., Hui-Zhao, Wang D., Jiang G., Wang J., Zhang G. 2002. Molecular cloning, identification and analysis of lung squamous cell carcinoma-related genes. Lung Cancer. 38(3), 235–241.

    Article  PubMed  Google Scholar 

  13. Liu Y., Zhu X., Zhu J., Liao S., Tang Q., Liu K., Guan X., Zhang G., Feng Z. 2007. Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray. Oncol. Rep. 18(4), 943–951.

    PubMed  Google Scholar 

  14. Sambrook J., Frisch E.F., Maniatis T. 1989. Molecular Cloning. A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Lab. Press.

  15. Zhu Y.Y., Machleder E.M., Chenchik A., Li R., Siebert P.D. 2001. Reverse transcriptase template switching: A SMART approach for full-length cDNA library construction. Biotechniques. 30(4), 892–897.

    CAS  PubMed  Google Scholar 

  16. Liu D.W., Chen S.T., Liu H.P. 2005. Choice of endogenous control for gene expression in non-small cell lung cancer. Eur. Respir. J. 26(6), 1002–1008.

    Article  PubMed  Google Scholar 

  17. Anedchenko E.A., Kiseleva N.P., Dmitriev A.A., Kisselev F.L., Zabarovsky E.R., Senchenko V.N. 2007. Tumor suppressor gene RBSP3 in cervical carcinoma: Copy number and transcription level. Mol. Biol. 41(1), 86–95.

    Article  CAS  Google Scholar 

  18. Glantz S.A. 1994. Primer of Biostatistics, 4th ed. N.Y.: McGraw-Hill Inc.

  19. Orino K., Watanabe K. 2008. Molecular, physiological and clinical aspects of the iron storage protein ferritin. Veterinary J. 178(2), 191–201.

    Article  CAS  Google Scholar 

  20. Milman N., Pedersen L.M. 2002. The serum ferritin concentration is a significant prognostic indicator of survival in primary lung cancer. Oncol. Rep. 9(1), 193–198.

    CAS  PubMed  Google Scholar 

  21. Singh K.J., Singh S.K., Suri A., Vijjan V., Goswami A.K., Khullar M. 2005. Serum ferritin in renal cell carcinoma: Effect of tumor size, volume grade, and stage. Indian J. Cancer. 42(4), 197–200.

    PubMed  Google Scholar 

  22. Kirkali Z., Esen A.A., Kirkali G., Guner G. 1995. Ferritin: A tumor marker expressed by renal cell carcinoma. Eur. Urol. 28(2), 131–134.

    CAS  PubMed  Google Scholar 

  23. Partin A.W., Criley S.R., Steiner M.S., Hsieh K., Simons J.W., Lumadue J., Carter H.B., Marshall F.F. 1995. Serum ferritin as a clinical marker for renal cell carcinoma: influence of tumor volume. Urology. 45(2), 211–217.

    Article  CAS  PubMed  Google Scholar 

  24. Kirkali Z., Guzelsoy M., Mungan M.U., Kirkali G., Yorukoglu K. 1999. Serum ferritin as a clinical marker for renal cell carcinoma: Influence of tumor size and volume. Urol. Int. 62(1), 21–25.

    Article  CAS  PubMed  Google Scholar 

  25. Sammarco M.C., Ditch S., Banerjee A., Grabczyk E. 2008. Ferritin L subunits are differentially regulated on a post-transcriptional level. J. Biol. Chem. 283(8), 4578–4587.

    Article  CAS  PubMed  Google Scholar 

  26. An H.J., Kim D.S., Park Y.K., Kim S.K., Choi Y.P., Kang S., Ding B., Cho N.H. 2006. Comparative proteomics of ovarian epithelial tumors. J. Proteome Res. 5(5), 1082–1090.

    Article  CAS  PubMed  Google Scholar 

  27. Wigle D.A., Tsao M., Jurisica I. 2004. Making sense of lung-cancer gene-expression profiles. Genome Biol. 5(2), 309.

    Article  PubMed  Google Scholar 

  28. Blackhall F.H., Wigle D.A., Jurisica I., Pintilie M., Liu N., Darling G., Johnston M.R., Keshavjee S., Waddell T., Winton T., Shepherd F.A., Tsao M.S. 2004. Validating the prognostic value of marker genes derived from a nonsmall cell lung cancer microarray study. Lung Cancer. 46(2), 197–204.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    A. V. Kudriavtseva, E. A. Anedchenko, N. Yu. Oparina, G. S. Krasnov, A. A. Dmitriev & V. N. Senchenko

  2. Blokhin Russian Cancer Research Center, Russian Academy of Sciences, Moscow, 115478, Russia

    I. B. Zborovskaya & T. T. Kondratjeva

  3. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    K. N. Kashkin, E. V. Vinogradova, M. V. Zinovyeva & E. P. Kopantsev

Authors
  1. A. V. Kudriavtseva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. A. Anedchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. Yu. Oparina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. S. Krasnov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. N. Kashkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. A. Dmitriev
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. B. Zborovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. T. T. Kondratjeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. E. V. Vinogradova
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. M. V. Zinovyeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. E. P. Kopantsev
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. V. N. Senchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. V. Kudriavtseva.

Additional information

Original Russian Text © A.V. Kudriavtseva, E.A. Anedchenko, N.Yu. Oparina, G.S. Krasnov, K.N. Kashkin, A.A. Dmitriev, I.B. Zborovskaya, T.T. Kondratjeva, E.V. Vinogradova, M.V. Zinovyeva, E.P. Kopantsev, V.N. Senchenko, 2009, published in Molekulyarnaya Biologiya, 2009, Vol. 43, No. 6, pp. 1044–1054.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kudriavtseva, A.V., Anedchenko, E.A., Oparina, N.Y. et al. Expression of FTL and FTH genes encoding ferritin subunits in lung and renal carcinomas. Mol Biol 43, 972–981 (2009). https://doi.org/10.1134/S0026893309060090

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0026893309060090

Key words

Search

Navigation