Abstract
Two novel strains of obligately alkaliphilic (pH 7.5–10.2, optimum pH 8.4–8.8) anaerobic spore-forming rod-shaped bacteria, Z-0511 and Z-7031, were isolated from enrichment cultures obtained from the iron-reducing (Lake Khadyn, Tyva) and cellulolytic (Lake Verkhnee Beloe, Buryatia) bacterial communities, respectively. The organisms ferment peptides and do not ferment proteins and amino acids, with the exception of histidine and glutamate utilized by strain Z-0511. The major fermentation products were acetate and propionate for strain Z-0511 and formate and acetate for strain Z-7031, respectively. Carbohydrates and fermentable organic acids could not serve as substrates, except for pyruvate in the case of strain Z-7031. Nitrogen and sulfur compounds were not utilized as electron acceptors by the strains grown on medium with yeast extract. Strain Z-0511 utilized fumarate, crotonate, and EDTA-Fe(III) as electron acceptors. Anthraquinone-2,6-disulfonate (quinone) and Mn(IV) were utilized by both strains, as well as amorphous ferric hydroxide (AFH), which was reduced to iron sesquioxides and magnetite. The presence of AFH stimulated growth; it enhanced the yield of the fermentation products and changed the quantitative ratios of these products. According to a phylogenetic analysis of the 16S rRNA gene sequences and the phenotypic characteristics of the new strains, they were classified as new species of the genus Natronincola, Natronincola ferrireducens sp. nov. Z-0511T (= VKM B-2402, = DSM 18346) and Natronincola peptidovorans sp. nov. Z-7031T (= VKM B-2503, = DSM 18979).
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Kevbrin, V.V., Zhilina, T.N., Rainey, F.A., and Zavarzin, G.A., Tindallia magadii gen. nov., sp. nov.: An Alkaliphilic Anaerobic Ammonifier from Soda Lake Deposits, Curr. Microbiol., 1998, vol. 37, pp. 94–100.
Garnova, E.S., Zhilina, T.N., Tourova, T.P., and Lysenko, A.M., Anoxynatronum sibiricum gen. nov., sp. nov., Alkaliphilic Saccharolytic Anaerobe from Cellulolytic Community of Nizhnee Beloe (Transbaikal Region), Extremophiles, 2003, vol. 7, pp. 213–220.
Blum, J.S., Bindi, A.B., Buzzelli, J., Stolz, J.F., and Oremland, R.S., Bacillus arsenicoselenatis, sp. nov., and Bacillus selenitireducens, sp. nov.: Two Haloalkaliphiles from Mono Lake, California That Respire Oxyanions of Selenium and Arsenic, Arch. Microbiol., 1998, vol. 171, pp. 19–30.
Pollock, J., Weber, K.A., Lack, J., Achenbach, L.A., Mormile, M.A., and Coates, J.D., Alkaline Iron (III) Reduction by a Novel Alkaliphilic, Halotolerant, Bacillus sp. Isolated from Salt Flat Sediments of Soap Lake, Appl. Microbiol. Biotechnol., 2007, vol. 77, pp. 927–934.
Gorlenko, V., Tsapin, A., Namsaraev, Z., Teal, T., Tourova, T., Engler, D., Mielke, R., and Nealson, K., Anaerobranca californiensis sp. nov., an Anaerobic, Alkalithermophilic, Fermentative Bacterium Isolated from a Hot Spring on Mono Lake, Int. J. Syst. Evol. Microbiol., 2004, vol. 54, pp. 739–743.
Ye, Q., Roh, Y., Carroll, S.L., Blair, B., Zhou, J., Zhang, C.L., and Fields, M.W., Alkaline Anaerobic Respiration: Isolation and Characterization of a Novel Alkaliphilic and Metal-Reducing Bacterium, Arch. Microbiol., 2004, vol. 70, pp. 5595–5602.
Zavarzina, D.G., Kolganova, T.V., Boulygina, E.S., Kostrikina, N.A., Tourova, T.P., and Zavarzin, G.A., Geoalkalibacter ferrihydriticus gen. nov. sp. nov., the First Alkaliphilic Representative of the Family Geobacteraceae, Isolated from a Soda Lake, Mikrobiologiya, 2006, vol. 76, no. 6, pp. 673–678 [Microbiology (Engl. Transl.), vol. 76, no. 6, pp. 673–682].
Zhilina, T.N., Kevbrin, V.V., Tourova, T.P., Lysenko, A.M., Kostrikina, N.A., and Zavarzin, G.A., Clostridium alkalicellum sp. nov., an Obligately Alkaliphilic Cellulolytic Bacterium from a Soda Lake in the Baikal Region, Mikrobiologiya, 2005, vol. 74, pp. 642–653 [Microbiology (Engl. Transl.), vol. 74, no. 5, pp. 557–566].
Kevbrin, V.V. and Zavarzin, G.A., Effect of Sulfur Compounds on the Growth of the Halophilic Homoacetic Bacterium Acetohalobium arabaticum, Mikrobiologiya, 1992, vol. 61, no. 5, pp. 812–817.
Stead, D.E., Sellwood, J.E., Wilson, J., and Viney, I., Evaluation of a Commercial Microbial Identification System Based on Fatty Acid Profiles for Rapid, Accurate Identification of Plant Pathogenic Bacteria, J. Appl. Bacteriol., 1992, vol. 72, pp. 315–321.
Lovley, D.R. and Phillips, E.J.P., Organic Matter Mineralization with the Reduction of Ferric Iron in Anaerobic Sediments, Appl. Environ. Microbiol., 1986, vol. 51, pp. 683–689.
Chistyakova, N.I., Rusakov, V.S., and Zavarzina, D.G., Mössbauer Study of Kinetics and Formation Conditions of Iron Minerals by Thermophilic Iron Reducers, Izv. Akad. Nauk, Ser. Fiz., 2003, vol. 67, no. 9, pp. 1354–1358.
Lane, D.J, 16S/23S rRNA Sequencing, Nucleic Acid Techniques in Bacterial systematics, Stackebrandt, E.. and Goodfellow, M., Eds., Chichester,: UK: John Wiley & Sons, 1991, pp. 115–177.
Collins, M.D., Lawson, P.A., Willems, A., Cordoba, J.J., Fernandez-Garayzabal, J., Garcia, P., Cia, J., Hippe, H., and Farrow, J.A.E., The Phylogeny of the Genus Clostridium: Proposal of Five New Genera and Eleven New Species Combination, Int. J. Syst. Bacteriol., 1994, vol. 44, pp. 812–826.
Stackebrandt, E. and Goebel, B.M., A Place for DNADNA Reassociation and 16S RRNA Sequence Analyses in the Present Species Definition in Bacteriology, Int. J. Syst. Bacteriol., 1994, vol. 44, pp. 846–849.
Stackebrandt, E., and Ebers, J., Taxonomic Parameters Revisited: Tarnished Gold Standards, Microbiol. Today vol. 33, pp. 152–155
Cato, E.P., George, W.L., and Finegold, S.M., Genus Clostridium, Bergey’s Manual of Systematic Bacteriology, vol. 2, Williams & Wilkins, 1986, pp. 1149–1168.
Andreesen, J.R., Gottschalk, G., and Schlegel, H.G., Clostridium formicoaceticum Nov. Spec. Isolation, Description, and Distinction from C. aceticum and C. thermoaceticum, Arch. Mikrobiol., 1970, vol. 72, pp. 154–174.
Zhilina, T.N., Detkova, E.N., Rainey, F.A., Osipov, G.A., Lysenko, A.M., Kostrikina, N.A., and Zavarzin, G.A., Natronoincola histidinovorans gen. nov., sp. nov., a New Alkaliphilic Acetogenic Anaerobe, Curr. Microbiol., 1998, vol. 37, pp. 177–185.
Pikuta, E.V., Hoover, R.B., Bej, A.K., Marsic, D., Detkova, E.N., Whitman, W.B., and Krader, P., Tindallia californiensis sp. nov., a New Anaerobic, Haloalkaliphilic, Spore-Forming Acetogen Isolated from Mono Lake in California, Exremophiles, 2003, vol. 7, pp. 327–334.
Zhilina, T.N., Zavarzina, D.G., Kolganova, T.V., Lysenko, A.M., Tourova, T.P., Alkaliphilus peptidofermentans sp. nov., a New Alkaliphilic Bacterial Soda Lake Isolate Capable of Peptide Fermentation and Fe(III) Reduction, Mikrobiologiya, 2009, Vol. 78, No. 4, pp. 496–505 [Microbiology (Engl. Transl.), vol. 78, no. 4, pp. 445–454].
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Original Russian Text © T.N. Zhilina, D.G. Zavarzina, G.A. Osipov, N.A. Kostrikina, T.P. Tourova, 2009, published in Mikrobiologiya, 2009, Vol. 78, No. 4, pp. 506–518.
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Zhilina, T.N., Zavarzina, D.G., Osipov, G.A. et al. Natronincola ferrireducens sp. nov., and Natronincola peptidovorans sp. nov., new anaerobic alkaliphilic peptolytic iron-reducing bacteria isolated from soda lakes. Microbiology 78, 455–467 (2009). https://doi.org/10.1134/S0026261709040092
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DOI: https://doi.org/10.1134/S0026261709040092