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Modeling of Hypercorticosteronemia in Rats Using Osmotic Pumps

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Abstract

The administration of the adrenal hormone corticosterone by injection or subcutaneous implantation of its modified-release dosage forms is often used to model situations associated with the effects of various stressors on the brain. The present methodological study investigated the possibility of using Alzet osmotic pumps (OPs) with a capacity of 200 µL and a release rate of 1 µL/h, loaded with corticosterone solutions of 25, 50 and 100 mg/mL or a vehicle (dimethyl sulfoxide/polyethylene glycol-400). The objective was to determine the optimal concentration of corticosterone solution loaded into osmotic pumps to create an increased level of the hormone in the rat blood and brain regions. An increase in blood corticosterone levels was only observed on   mg/mL (OP-100). Corticosterone accumulation in the frontal cortex, neocortex and hippocampus increased significantly by day 3 after implantation in the OP-100 group. Thus, OPs with a corticosterone solution in DMSO/PEG-400, at a hormone concentration of 100 mg/mL and release rate of 1 µL/h, can be used to create elevated corticosterone levels in the blood and brain regions of rats.

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Funding

This work was supported by the Russian Science Foundation, grant no. 20-65-47029.

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Authors and Affiliations

  1. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia

    M. V. Onufriev, Yu. V. Moiseeva & N. V. Gulyaeva

Authors
  1. M. V. Onufriev
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  2. Yu. V. Moiseeva
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  3. N. V. Gulyaeva
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Contributions

Conceptualization and experimental design (N.V.G., M.V.O.); data collection and treatment (Yu.V.M.); writing and editing the manuscript (M.V.O., N.V.G.).

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Correspondence to M. V. Onufriev or N. V. Gulyaeva.

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The authors declare that they have no conflicts of interest.

Additional information

Translated by A. Polyanovsky

Russian Text © The Author(s), 2022, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2022, Vol. 108, No. 11, pp.1542–1550https://doi.org/10.31857/S0869813922110085.

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Onufriev, M.V., Moiseeva, Y.V. & Gulyaeva, N.V. Modeling of Hypercorticosteronemia in Rats Using Osmotic Pumps. J Evol Biochem Phys 58, 1987–1993 (2022). https://doi.org/10.1134/S0022093022060266

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