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Somatostatin-Expressing Neurons in the Tuberal Region of Rat Hypothalamus during Aging

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Abstract

The location and neurochemical composition of somatostatin (SOM)-immunoreactive (ir) neurons in the tuberal region of the rat hypothalamus were studied using immunohistochemical method in young (2–3-month-old), adult (1-year-old), and aged (2–2.5-year-old) male and female rats. The highest percentage of SOM-ir neurons was found in the arcuate nucleus (AN) and ventrolateral part of the ventromedial nucleus (VMNvl). A smaller part of them was observed in the dorsomedial (DMN) and medial tuberal (MTu) nuclei, as well as in the VMN shell. Single SOM-ir neurons occurred in the dorsomedial part of the ventromedial nucleus (VMNdm), periventricular (PeV) and perifornical (PeF) nuclei. No age-related changes were found in the number of SOM-ir neurons. The percentage of SOM-ir neurons colocalizing calbindin (CB), neuronal nitric oxide synthase (nNOS) and neuropeptide Y (NPY) increased in the AN during aging. In the VMNvl, from one third to one half of SOM-ir neurons in all rats colocalized nNOS, and only from 7.9 to 18.6% of them colocalized CB and NPY. In the DMN of males, a significantly higher percentage of SOM-ir neurons colocalizing NPY was observed in all age groups when compared with females. Thus, aging is accompanied by neurochemical changes in the SOM-ergic system of the tuberal hypothalamic nuclei, mainly in the arcuate nuclei.

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Funding

This work was supported by the Russian Science Foundation (grant no. 19-15-00039).

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Authors and Affiliations

  1. Yaroslavl State Medical University, Yaroslavl, Russia

    P. A. Vishnyakova, K. Yu. Moiseev, V. V. Porseva, L. G. Pankrasheva & P. M. Masliukov

  2. H.M. Berbekov Kabardino-Balkarian State University, Nalchik, Russia

    A. F. Budnik

  3. St. Petersburg State University, St. Petersburg, Russia

    A. D. Nozdrachev

Authors
  1. P. A. Vishnyakova
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  2. K. Yu. Moiseev
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  3. V. V. Porseva
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  4. L. G. Pankrasheva
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  5. A. F. Budnik
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  6. A. D. Nozdrachev
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  7. P. M. Masliukov
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Contributions

Conceptualization and experimental design (P.M.M., A.D.N.); data collection (P.A.V., K.Yu.M.); data processing (A.F.B., V.V.P., L.G.P.); writing and editing the manuscript (P.M.M., V.V.P.).

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Correspondence to P. M. Masliukov.

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The authors declare that they have neither evident nor potential conflict of interest related to the publication of this article.

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Translated by A. Polyanovsky

Russian Text © The Author(s), 2021, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2021, Vol. 107, No. 12, pp. 1530–1541https://doi.org/10.31857/S0869813921120116.

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Vishnyakova, P.A., Moiseev, K.Y., Porseva, V.V. et al. Somatostatin-Expressing Neurons in the Tuberal Region of Rat Hypothalamus during Aging. J Evol Biochem Phys 57, 1480–1489 (2021). https://doi.org/10.1134/S0022093021060247

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