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Activation of heart contractility of the edible snail H. pomatia by thrombin. Study of the role of cAMP

  • Comparative and Ontogenic Physiology
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Abstract

Thrombin acts on mammalian cells through the specific, so-called protease-activated receptors (PARs). The thrombin action is mediated via three out of four known types of these receptors—PAR1,3,4. Mammalian thrombin receptors, apart from performance of other functions, control cardiac and vascular contractility. It is not known whether receptors of such kind exist in invertebrate animals. In the present work we have showed for the first time that thrombin in the concentration range of 0.01–1 units/ml increases amplitude of contractions of the isolated heart ventricle of the edible snail Helix pomatia. Its effect is reproduced by peptide ligands of receptors PAR1 and PAR4 that have sequences Ser-Phe-Leu-Leu-Arg-Asn (SFLLRN) and Glu-Tyr-Pro-Gly-Lys-Phe (QYPGKF), respectively. A potent activator of cardiac contractility of H. pomatia is serotonin. A comparative study of the mechanisms of action of serotonin and thrombin on the edible snail heart was carried out. cAMP participates in transduction of signal from serotonin receptors. On the membrane preparation from the H. pomatia heart, it was shown that thrombin and peptide ligands PAR1 and PAR4, unlike serotonin, did not increase adenylyl cyclase activity. Thus, mechanism of activation of cardiac contractility of H. pomatia by thrombin differs from that of serotonin. It is suggested that molluscs have receptors homologous to protease-activated mammalian receptors.

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Authors and Affiliations

  1. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Vavilova str. 26, Moscow, Russia

    V. G. Solomonova, P. P. Avdonin, E. S. Vinichenko, I. F. Sukhanova & P. V. Avdonin

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  1. V. G. Solomonova
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  2. P. P. Avdonin
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  3. E. S. Vinichenko
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  4. I. F. Sukhanova
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  5. P. V. Avdonin
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Original Russian Text © V. G. Solomonova, P. P. Avdonin, E. S. Vinichenko, I. F. Sukhanova, and P. V. Avdonin, 2007, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2007, Vol. 43, No. 1, pp. 32–38.

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Solomonova, V.G., Avdonin, P.P., Vinichenko, E.S. et al. Activation of heart contractility of the edible snail H. pomatia by thrombin. Study of the role of cAMP. J Evol Biochem Phys 43, 35–42 (2007). https://doi.org/10.1134/S0022093007010036

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  • DOI: https://doi.org/10.1134/S0022093007010036

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