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Lipid-induced changes in protein conformation as a means to regulate the immunogenicity of antigens incorporated in tubular immunostimulating complexes

  • Molecular Biophysics
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Abstract

Nanoparticles of tubular immunostimulating complexes (TI-complexes), which consist of the glycolipid monogalactosyldiacylglycerol (MGDG) from marine macrophytes (macroalgae and seagrasses), the triterpene glycoside cucumarioside A2-2 from the holothurian Cucumaria japonica, and cholesterol, are a promising adjuvant carrier of antigens for modern subunit vaccines. MGDG provides a lipid matrix for the antigen incorporated in TI-complexes. This paper discusses the manner in which the physicochemical properties of MGDGs isolated from different marine macrophyte species affect the conformation of two model protein antigens (Yersinia pseudotuberculosis OmpF-like porin (YOmpF) and recombinant influenza virus hemagglutinin) incorporated in TI-complexes and how the modulating effect of MGDG may be used to improve the efficacy of vaccine preparations.

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Abbreviations

MGDG:

monogalactosyldiacylglycerol

TI-complex:

tubular immunostimulating complex

YOmpF:

Yersinia pseudotuberculosis OmpF-like porin

PUFA:

polyunsaturated fatty acid

DSC:

differential scanning calorimetry

HA0:

recombinant uncleaved hemagglutinin monomer

GM-CSF:

granulocyte–macrophage colony-stimulating factor

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Authors and Affiliations

  1. Far Eastern Federal University, ul. Sukhanova 8, Vladivostok, 690950, Russia

    N. M. Sanina, N. S. Vorobieva, L. A. Davydova & E. Ya. Kostetsky

  2. Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, pr. Stoletiya Vladivostoka 159, Vladivostok, 690022, Russia

    O. D. Novikova & O. Yu. Portniagina

  3. Universidad de Salamanca, Plaza Doctores de la Reina s/n, Salamanca, 37007, Spain

    V. L. Shnyrov

Authors
  1. N. M. Sanina
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  2. N. S. Vorobieva
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  3. O. D. Novikova
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  4. O. Yu. Portniagina
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  5. L. A. Davydova
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  6. V. L. Shnyrov
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  7. E. Ya. Kostetsky
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Corresponding author

Correspondence to N. M. Sanina.

Additional information

Original Russian Text © N.M. Sanina, N.S. Vorobieva, O.D. Novikova, O.Yu. Portniagina, L.A. Davydova, V.L. Shnyrov, E.Ya. Kostetsky, 2016, published in Biofizika, 2016, Vol. 61, No. 3, pp. 455–463.

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Sanina, N.M., Vorobieva, N.S., Novikova, O.D. et al. Lipid-induced changes in protein conformation as a means to regulate the immunogenicity of antigens incorporated in tubular immunostimulating complexes. BIOPHYSICS 61, 380–386 (2016). https://doi.org/10.1134/S0006350916030155

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  • DOI: https://doi.org/10.1134/S0006350916030155

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