Abstract
The significant destructive changes in ultrastructure of hepatocytes from laboratory mice kept in different vivariums in Moscow and fed with dry laboratory animal diets acquired from different domestic manufacturers that were not standardized for initial products were demonstrated using electron microscopy. Furthermore, disruption in the ultrastructure of liver parenchymal cells occurred regardless of the animal status (SPF or conventional), conditions of various vivariums, as well as the feed manufacturer. At the same time, studies on ultrastructure of liver hepatocytes from mice kept in the Charles River Laboratory facilities in Germany and fed with the Altromin Spezialfutter laboratory animal diet (GmbH & Co., Germany) that was produced using quality control of ingredients did not reveal destructive changes in the internal ultrastructure of hepatocytes. However, if these mice were later fed with the food produced in local manufactures, changes in the structure of liver cells developed after 2 months. Thus, feeding with dry diet from the domestic producers of an unspecified composition causes significant changes in the ultrastructure of hepatocytes in control animals, reflecting the development of some pathological processes in the body.
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Abbreviations
- agEPR:
-
agranular EPR
- EPR:
-
endoplasmic reticulum
- grEPR:
-
granular EPR
- IVC:
-
individually ventilated cage
- NAFLD:
-
nonalcoholic fatty liver disease
- SPF status:
-
specific-pathogen-free animals
- VEC:
-
Complex of the Animal Facilities and the Research Unit Vivarium, Institute of Mitoengineering of Lomonosov Moscow State University
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Funding
This work was supported by the Russian Foundation for Basic Research (project no. 19-04-00578) and performed with support of TSITIS, Russia (project no. AAAA-A19-119012490166-2).
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The authors declare no conflicts of interests. All applicable international, national and/or institutional principles for the care and use of animals were followed.
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Vays, V.B., Vangeli, I.M., Averina, O.A. et al. Ultrastructure of Hepatocytes from Laboratory Mice Fed a Standard Dry Laboratory Animal Diet. Biochemistry Moscow 85, 1082–1092 (2020). https://doi.org/10.1134/S0006297920090084
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DOI: https://doi.org/10.1134/S0006297920090084