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Effect of low temperature on globin expression, respiratory metabolic enzyme activities, and gill structure of Litopenaeus vannamei

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Abstract

Low temperature frequently influences growth, development, and even survival of aquatic animals. In the present study, physiological and molecular responses to low temperature in Litopenaeus vannamei were investigated. The cDNA sequences of two oxygen-carrying proteins, cytoglobin (Cygb) and neuroglobin (Ngb), were isolated. Protein structure analysis revealed that both proteins share a globin superfamily domain. Real-time PCR analysis indicated that Cygb and Ngb mRNA levels gradually increased during decrease in temperatures from 25 to 15°C and then decreased at 10°C in muscle, brain, stomach, and heart, except for a continuing increase in gills, whereas they showed a different expression trend in the hepatopancreas. Hemocyanin concentration gradually reduced as the temperature decreased. Moreover, the activities of respiratory metabolic enzymes including lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were measured, and it was found that LDH activity gradually increased while SDH activity decreased after low-temperature treatment. Finally, damage to gill structure at low temperature was also observed, and this intensified with further decrease in temperature. Taken together, these results show that low temperature has an adverse influence in L. vannamei, which contributes to systematic understanding of the adaptation mechanisms of shrimp at low temperature.

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Abbreviations

Cygb:

cytoglobin

Hb:

hemoglobin

LDH:

lactate dehydrogenase

Mb:

myoglobin

Ngb:

neuroglobin

ROS:

reactive oxygen species

SDH:

succinate dehydrogenase

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Authors and Affiliations

  1. Ministry of Education, Huazhong Agricultural University, College of Fishery, Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction, 430070, Wuhan, PR China

    Meng Wu, Nan Chen, Chun-Xiao Huang, Yan He & Huan-Ling Wang

  2. Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, PR China

    Yong-Zhen Zhao & Huan-Ling Wang

  3. Guangxi Academy of Fishery Sciences, 530021, Nanning, PR China

    Xiao-Han Chen & Xiu-Li Chen

Authors
  1. Meng Wu
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  2. Nan Chen
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  3. Chun-Xiao Huang
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  4. Yan He
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  5. Yong-Zhen Zhao
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  7. Xiu-Li Chen
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Correspondence to Xiu-Li Chen or Huan-Ling Wang.

Additional information

Published in Russian in Biokhimiya, 2017, Vol. 82, No. 7, pp. 1097–1106.

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Wu, M., Chen, N., Huang, CX. et al. Effect of low temperature on globin expression, respiratory metabolic enzyme activities, and gill structure of Litopenaeus vannamei . Biochemistry Moscow 82, 844–851 (2017). https://doi.org/10.1134/S0006297917070100

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  • DOI: https://doi.org/10.1134/S0006297917070100

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