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Identification of distinct lipopolysaccharide patterns among Yersinia enterocolitica and Y. enterocolitica-like bacteria

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Abstract

The lipopolysaccharide (LPS) of strains representing various serotypes of Yersinia enterocolitica and Y. enterocolitica-like bacteria was studied by deoxycholate-PAGE and silver staining analysis. Four main types of LPS were detected based on the O-polysaccharide (O-PS): (i) LPS with homopolymeric O-PS, (ii) LPS with ladder-forming heteropolymeric O-PS, (iii) LPS with single-length O-PS, and (iv) semi-rough LPS without O-PS. Within the first three types, several subvariants were detected. Selected serotypes representing all above LPS types are sensitive to bacteriophage ϕR1-37 indicating that they share the phage receptor, a hexasaccharide called outer core in Y. enterocolitica O:3. Whereas phage ϕR1-37-resistant mutants of homopolymeric O-PS have lost only the outer core, those of ladder-forming or single-length O-PS have lost also the O-PS suggesting that in the latter ones the outer core is bridging between O-PS and lipid A-core. This work forms a basis of further structural, biochemical and genetic studies of these LPSs.

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Abbreviations

DOC:

deoxycholate

IC:

inner core

LPS:

lipopolysaccharide

OC:

outer core

O-PS:

O-polysaccharide

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Authors and Affiliations

  1. Department of Bacteriology and Immunology, Haartman Institute, Infection Biology Research Program, University of Helsinki, PO Box 21, 00014, Helsinki, Finland

    M. Skurnik & S. Toivonen

  2. Helsinki University Central Hospital Laboratory Diagnostics, Helsinki, Finland

    M. Skurnik

Authors
  1. M. Skurnik
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  2. S. Toivonen
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Correspondence to M. Skurnik.

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Published in Russian in Biokhimiya, 2011, Vol. 76, No. 7, pp. 1006–1015.

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Skurnik, M., Toivonen, S. Identification of distinct lipopolysaccharide patterns among Yersinia enterocolitica and Y. enterocolitica-like bacteria. Biochemistry Moscow 76, 823–831 (2011). https://doi.org/10.1134/S0006297911070133

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  • DOI: https://doi.org/10.1134/S0006297911070133

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