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Biological properties and structure of the lipopolysaccharide of a vaccine strain of Francisella tularensis generated by inactivation of a quorum sensing system gene qseC

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Abstract

A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.

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Abbreviations

CFU:

colony-forming unit

dHexN:

deoxyhexosamine

ESI FT ICR:

electrospray ionization Fourier-transform ion-cyclotron resonance

GalNAcAN:

2-acetamido-2-deoxygalacturonamide

Hex:

hexose

HexN:

hexosamine

HexNAN:

hexosaminuronamide

Kdo:

3-deoxy-D-manno-oct-2-ulosonic acid

LPS:

lipopolysaccharide

Mr:

molecular weight

QS:

quorum sensing

QuiNAc:

2-acetamido-2,6-dideoxyglu-cose

Qui4N:

4-amino-4,6-dideoxyglucose

Qui4NFo:

4-form-amido-4,6-dideoxyglucose

3HO18:0:

3-hydroxyoctadecanoic acid

16:0:

hexadecanoic (palmitic) acid

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Authors and Affiliations

  1. State Research Center for Applied Microbiology and Biotechnology, 142279, Obolensk, Moscow Region, Russia

    A. N. Mokrievich, M. E. Platonov, G. M. Vakhrameeva, R. Z. Shaikhutdinova, R. I. Mironova, I. V. Bakhteeva, G. M. Titareva, T. B. Kravchenko, T. I. Kombarova, V. M. Pavlov & I. A. Dyatlov

  2. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 119991, Moscow, Russia

    A. N. Kondakova & Yu. A. Knirel

  3. Research Center Borstel, Center for Medicine and Biosciences, D-23485, Borstel, Parkallee 22, Germany

    A. N. Kondakova & B. Lindner

  4. Centre de Recherches du Service de Sante des Armees, BP 87, Avenue des Maquis du Gresivaudan 24, F-38702, La Tronche Cedex, France

    E. Valade, D. Blaha & D. Vidal

Authors
  1. A. N. Mokrievich
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  2. A. N. Kondakova
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  3. E. Valade
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  4. M. E. Platonov
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  5. G. M. Vakhrameeva
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  6. R. Z. Shaikhutdinova
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  7. R. I. Mironova
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  8. D. Blaha
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  9. I. V. Bakhteeva
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  10. G. M. Titareva
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  11. T. B. Kravchenko
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  12. T. I. Kombarova
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  13. D. Vidal
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  14. V. M. Pavlov
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  15. B. Lindner
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  16. I. A. Dyatlov
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  17. Yu. A. Knirel
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Corresponding author

Correspondence to A. N. Kondakova.

Additional information

Published in Russian in Biokhimiya, 2010, Vol. 75, No. 4, pp. 539–548.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM09-288, February 14, 2010.

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Mokrievich, A.N., Kondakova, A.N., Valade, E. et al. Biological properties and structure of the lipopolysaccharide of a vaccine strain of Francisella tularensis generated by inactivation of a quorum sensing system gene qseC . Biochemistry Moscow 75, 443–451 (2010). https://doi.org/10.1134/S0006297910040073

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  • DOI: https://doi.org/10.1134/S0006297910040073

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