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Proteinase inhibitors from the tropical sea anemone Radianthus macrodactylus: Isolation and characteristic

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Abstract

Two new serine proteinase inhibitors (RmIn I and RmIn II) from the tropical sea anemone Radianthus macrodactylus have been isolated and characterized. The purification procedure includes polychrome-1 hydrophobic chromatography, Superdex™ Peptide 10/30 FPLC, and Nucleosil C18 reverse-phase HPLC. The molecular masses of RmIn I, RmIn II, and the complexes RmIn II/trypsin and RmIn I,II/α-chymotrypsin have been determined. The K i values of RmIn I and RmIn II for trypsin and α-chymotrypsin have been determined. The polypeptides RmIn I and RmIn II are shown to be nontoxic and to exhibit antihistamine activity. The N-terminal amino acid sequences of RmIn I (GICSEPIVVGPCKAG-) and RmIn II (GSTCLEPKVVGPCKA-) have been determined. A high homology of the amino acid sequences is demonstrated for the proteinase inhibitors produced by such evolutionarily distant species as coelenterates, reptiles, and mammals.

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Abbreviations

BAPNA:

N-benzoyl-D,L-arginine p-nitroanilide

BSA:

bovine serum albumin

BTEE:

N-benzoyl-L-tyrosine ethyl ester

HPLC:

high performance liquid chromatography

TFA:

trifluoroacetic acid

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100-let Vladivostoku 159, 690022, Vladivostok, Russia

    I. N. Sokotun, A. P. Il’ina, M. M. Monastyrnaya, E. V. Leychenko, A. A. Es’kov, S. D. Anastuk & E. P. Kozlovskaya

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  1. I. N. Sokotun
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  2. A. P. Il’ina
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  3. M. M. Monastyrnaya
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  4. E. V. Leychenko
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  5. A. A. Es’kov
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  6. S. D. Anastuk
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  7. E. P. Kozlovskaya
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Correspondence to I. N. Sokotun.

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Original Russian Text © I. N. Sokotun, A. P. Il’ina, M. M. Monastyrnaya, E. V. Leychenko, A. A. Es’kov, S. D. Anastuk, E. P. Kozlovskaya, 2007, published in Biokhimiya, 2007, Vol. 72, No. 3, pp. 368–374.

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Sokotun, I.N., Il’ina, A.P., Monastyrnaya, M.M. et al. Proteinase inhibitors from the tropical sea anemone Radianthus macrodactylus: Isolation and characteristic. Biochemistry Moscow 72, 301–306 (2007). https://doi.org/10.1134/S0006297907030078

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  • DOI: https://doi.org/10.1134/S0006297907030078

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