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Antioxidative Compounds from the Secreted Metabolome of Strain ‘Mucor irregularis Isolate Dro2’—an Endophyte of the Carnivorous Plant Drosera burmannii

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Abstract

Endophytic fungi isolated from the insectivorous plant Drosera burmannii were tested for their antioxidant potential. The isolate Dro2 was found to have superlative antioxidant activity of the culture broth, scavenging 57.6 ± 0.2% of 2,2-diphenyl-1-picrylhydrazyl free radical. 5.8S rDNA homology led to identification of the isolate as a new strain and it has been named Mucor irregularis isolate Dro2. Optimization studies revealed ethyl acetate to be optimum for extraction of the antioxidant compounds. The crude solvent extract showed 89.6% 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging activity with respect to 86.1% ABTS radical reducing antioxidant power of ascorbic acid. The sample exhibited 824.0 ± 25.5 µM reducing ability in assay, compared to 1204.0 ± 17.8 µM for ascorbic acid. The sample extract displayed IC50DPPH of 53.6 µg/mL. The crude extract was further purified through various chromatography techniques and GC-MS was performed on the purified active fractions to ascertain the nature and identify compounds conferring antioxidant potential to the endophytic fungal isolate. The active fractions were found to comprise multitudinous compounds with varied biological activity. Antioxidant compounds, such as 2,4-di-tert-butylphenol, myristic acid, pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-, palmitic acid, were found in significant abundance. This study substantiates the candidature of the endophytic fungus Mucor irregularis isolate Dro2 as an industrially capable source for obtaining bioactive compounds, particularly antioxidant compounds.

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Funding

Authors are grateful to the Council of Scientific and Industrial Research, India, for financial support in the form of junior research fellowship to SM.

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Authors and Affiliations

  1. Microbiology and Microbial Biotechnology Laboratory, Department of Botany and Forestry, Vidyasagar University, 721102, Midnapore, West Bengal, India

    S. Mandal, S. Maity & D. Banerjee

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  1. S. Mandal
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  2. S. Maity
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  3. D. Banerjee
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Contributions

Conceptualization, experiments, data collection and analysis were performed by S. Mandal. S. Maity helped in chromatography, HPLC and GC-MS studies. Manuscript was prepared by S. Mandal. Insightful comments were received from S. Maity and D. Banerjee which helped in drafting the final version of the manuscript. All the research work was supervised by D. Banerjee.

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Correspondence to D. Banerjee.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Mandal, S., Maity, S. & Banerjee, D. Antioxidative Compounds from the Secreted Metabolome of Strain ‘Mucor irregularis Isolate Dro2’—an Endophyte of the Carnivorous Plant Drosera burmannii. Appl Biochem Microbiol 57 (Suppl 1), S88–S97 (2021). https://doi.org/10.1134/S0003683821100069

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