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Nitric-Oxide Synthase Activity in the Photomorphogenesis of Neurospora сrassa

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Abstract

Nitric-oxide synthase activity was detected in Neurospora crassa cells with the measurement of the conversion of 3H-L-arginine to 3H-L-citrulline. Some characteristics of this activity (sensitivity to calcium ions, action of specific enzyme inhibitors, and western blot analysis) were similar to those of an inducible enzyme found in mammals. According to western blotting, the molecular weight of NO synthase was around 130 kDa. No light-dependent changes in the specific activity of NO synthase were revealed in the photocarotenogenesis and photoconidiation of N. сrassa.

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Authors and Affiliations

  1. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia

    S. Yu. Filippovich, G. P. Bachurina & M. S. Kritsky

  2. Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia

    M. V. Onufriev

  3. Serbsky Federal Medical Research Center of Psychiatry and Narcology of the Ministry of Health of the Russian Federation, 119034, Moscow, Russia

    D. I. Peregud

Authors
  1. S. Yu. Filippovich
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  2. M. V. Onufriev
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  3. D. I. Peregud
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  4. G. P. Bachurina
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  5. M. S. Kritsky
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Correspondence to S. Yu. Filippovich.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by A. Boutanaev

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Filippovich, S.Y., Onufriev, M.V., Peregud, D.I. et al. Nitric-Oxide Synthase Activity in the Photomorphogenesis of Neurospora сrassa. Appl Biochem Microbiol 56, 446–452 (2020). https://doi.org/10.1134/S0003683820040043

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  • DOI: https://doi.org/10.1134/S0003683820040043

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