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Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices

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Abstract

Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.

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Authors and Affiliations

  1. Institute of Laser and Information Technologies, Department of Advanced Laser Technology, Russian Academy of Sciences, Troitsk, Moscow, 108840, Russia

    P. S. Timashev, K. N. Bardakova, N. V. Minaev, A. A. Akovantseva, A. V. Koroleva, B. N. Chichkov & V. N. Bagratashvili

  2. Enikolopov Institute of Synthetic Polymeric Materials, Russian Academy of Sciences, Moscow, 117393, Russia

    T. S. Demina & T. A. Akopova

  3. Nizhny Novgorod State Medical Academy, Ministry of Healthcare of the Russian Federation, Nizhny Novgorod, 603005, Russia

    T. A. Mishchenko, E. V. Mitroshina & I. V. Mukhina

  4. Burdenko Research Institute of Neurosurgery, Ministry of Healthcare of the Russian Federation, Moscow, 125047, Russia

    D. S. Asyutin, L. F. Pimenova & N. A. Konovalov

  5. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, 119991, Russia

    A. B. Solov’eva

  6. Institute of Biology and Biomedicine, Lobachevskii National Research State University, Nizhny Novgorod, 603950, Russia

    M. V. Vedunova

Authors
  1. P. S. Timashev
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  2. K. N. Bardakova
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  3. N. V. Minaev
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  4. T. S. Demina
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  5. T. A. Mishchenko
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  6. E. V. Mitroshina
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  7. A. A. Akovantseva
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  8. A. V. Koroleva
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  9. D. S. Asyutin
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  10. L. F. Pimenova
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  11. N. A. Konovalov
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  12. T. A. Akopova
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  13. A. B. Solov’eva
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  14. I. V. Mukhina
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  15. M. V. Vedunova
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  16. B. N. Chichkov
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  17. V. N. Bagratashvili
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Corresponding author

Correspondence to P. S. Timashev.

Additional information

Original Russian Text © P.S. Timashev, K. N Bardakova, N.V. Minaev, T.S. Demina, T.A. Mishchenko, E.V. Mitroshina, A.A. Akovantseva, A.V. Koroleva, D.S. Asyutin, L.F. Pimenova, N.A. Konovalov, T.A. Akopova, A.B. Solov’eva, I.V. Mukhina, M.V. Vedunova, B.N. Chichkov, V.N. Bagratashvilі, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 5, pp. 495–503.

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Timashev, P.S., Bardakova, K.N., Minaev, N.V. et al. Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices. Appl Biochem Microbiol 52, 508–514 (2016). https://doi.org/10.1134/S0003683816050161

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  • DOI: https://doi.org/10.1134/S0003683816050161

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