Abstract
The physicochemical and enzymatic properties of hybrid analogues of the Brevundimonas diminuta Gl7ACA-acylase (BrdGlA), containing the N-terminal chitin-binding domain of the bacterial chitinase (BrdGlA/NmChBD) or the C-terminal oligohistidine sequence (BrdGlA/H), were studied. An enhanced thermostability level of BrdGlA/NmChBD could suggest the stabilizing effect of the chitin-binding domain. An analysis of pH profiles of the enzymatic activity of recombinat BrdGlA analogues did not reveal significant differences: the catalytic activity of both variants changed slightly in the interval of pH values from 6.0 to 9.0 but drastically decreased at lower pH values. Both analogues demonstrated similar sensitivity towards denaturing agents: addition of 2.0 M of guanidine chloride resulted in the complete inactivation of both enzymes. A scheme was developed for obtaining isolated recombinant α- and β-subunits of BrdGLA. In vitro enzyme reconstructions indicated that the α-subunit was necessary for the formation of a correct spatial structure of the β-subunit and for the formation of a functionally active enzyme.
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Barber, M.S., Giesecke, U., Reichert, A., and Minas, W., Adv. Biochem. Eng. Biotechnol., 2004, vol. 88, pp. 179–215.
Kumar, K.K., Sudhakaran, V., Deshpande, B.S., Ambedkar, S.S., and Shewale, J.G., Hindustan Antibiot. Bull., 1993, vol. 35, pp. 111–125.
Aramori, I., Fukagawa, M., Tsumura, M., Iwami, M., Ono, H., Ishtani, Y., Hiroshi, K., Kojo, H., Kohsaka, M., Udea, Y., and Imenaka, H., J. Ferment. Bioeng., 1992, vol. 73, pp. 185–192.
Aramori, I., Fukagawa, M., Tsumura, M., Ono, H., Ishtani, Y., Kojo, H., Kohsaka, M., Udea, Y., and Imenaka, H., Eur. J. Biochem., 1995, vol. 230, pp. 773–778.
Deshpande, B.S., Ambedkar, S.S., Sudhakaran, V.K., and Shewale, J.G., J. Microbiol. Biotechnol., 1994, vol. 10, pp. 129–138.
Ishii, Y., Saito, Y., Fujimura, T., Isogai, T., Kojo, H., Yashimata, M., Niwa, M., and Kohsaka, M., J. Ferment. Bioeng., 1994, vol. 77, pp. 591–597.
Khatuntseva, S.A., Eldarov, M.A., Redo, V.A., and Skryabin, K.G., J. Biotechnol., 2008, vol. 133, pp. 123–126.
Li, Y., Jiang, W., Yang, Y., Zhao, G., and Wang, E., Protein Expr. Purif., 1998, vol. 12, pp. 233–238.
Luo, H., Yu, H., Li, Q., and Shen, Z., Tsinghua Scie. Technol., 2005, vol. 10, pp. 529–534.
Ledent, P., Duez, C., Vanhove, M., Lejeune, A., Fonze, E., and Charlier, P., FEBS Lett., 1997, vol. 413, pp. 194–196.
Zheng, H., Chena, J., Sua, L., Zhaob, Y., Yang, Y., Zenge, H., Xue, G., Yang, S., and Jiang, W., Enzyme Microb. Technol., 2007, vol. 41, pp. 474–479.
Wang, E.D., Zheng, Y.G., Li, Y., Jiang, W.H., and Yang, Y.L., Acta Biochim. Biophys. Sin., 2002, vol. 34, pp. 526–531.
Li, Y., Jiang, W., Yang, Y., Zhao, G., and Wang, E., Protein Expr. Purif., 2002, vol. 12, pp. 233–238.
Lee, Y.S., Kim, H.W., and Park, S.S., J. Biol. Chem., 2000, vol. 275, pp. 39200–39206.
Friehs, K. and Reardon, K.F., Adv. Biochem. Eng. Biotechnol., 1993, vol. 48, pp. 53–77.
Vethanayagam, J. and Flower, A., Microbial Cell Factories, 2005, vol. 4, p. 3.
Kim, Y., Yoon, K., Khang, Y., Turley, S., and Hol, W.G., Structure. Fold. Des., 2000, vol. 8, pp. 1059–1068.
Khatuntseva, S.A., El’darov, M.A., Lopatin, S.A., Zeinalov, O.A., and Skryabin, K.G., Appl. Biochem. Microbiol., 2007, vol. 43, no. 4, pp. 414–421.
Studier, F., Protein Expr. Purif., 2005, vol. 41, pp. 207–234.
Balasingham, K., Warburton, D., Dunnill, P., and Lilly, M.D., Biochim. Biophys. Acta, 1972, vol. 276, pp. 250–256.
Laemli, U.K., Nature, 1970, vol. 227, pp. 680–685.
Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.
Battistel, E., Bianchi, D., Bortolo, R., and Bonoldi, L., Appl. Biochem. Biotechnol., 1998, vol. 69, pp. 53–67.
Ishiye, M. and Niwa, M., Biochim. Biophys. Acta, 2002, vol. 1132, pp. 233–239.
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Original Russian Text © S.A. Zakirova, T.V. Mikhailova, M.A. Eldarov, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 6, pp. 554–560.
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Zakirova, S.A., Mikhailova, T.V. & Eldarov, M.A. Peculiarities of the Brevundimonas diminuta Gl7ACA-acylase quaternary structure formation and obtaining stable enzyme analogues. Appl Biochem Microbiol 49, 549–554 (2013). https://doi.org/10.1134/S0003683813060185
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DOI: https://doi.org/10.1134/S0003683813060185