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Ouabain-dependent activation of protein kinase ERK1/2 and the transcription factor STAT3 in carcinoma A431 cells

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

The effects of ouabain (10−7−10−3 M) on Rb+ (K+ analog) entry into the cell, intracellular content of K+ and Na+, tyrosine phosphorylation of the transcription factors STAT1 and STAT3, protein kinase ERK1/2 and the epidermal growth factor (EGF) receptor in human epidermoid carcinoma cell line A431 were studied. Ouabain (10−5−10−7 M) enhanced the tyrosine phosphorylation of ERK1/2, STAT3 (but not STAT1), and the EGF receptor. The activating effect on signaling molecules was observed only at low concentrations of ouabain, not affecting the Na+/K+ pump transport activity and intracellular content of Na+ and K+. The Src kinase inhibitor CGP77675 blocked the activating effect of ouabain on the phosphorylation of the EGF receptor, ERK1/2, and STAT3. These findings suggest that the EGF receptor and Src kinases are involved in the ouabain-induced activation of MAPK and STAT signaling pathways in A431 cells.

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Authors and Affiliations

  1. Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, St. Petersburg, Russia

    I. V. Epifantseva, T. A. Vinogradova & I. I. Marakhova

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  1. I. V. Epifantseva
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  2. T. A. Vinogradova
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  3. I. I. Marakhova
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Original Russian Text © I.V. Epifantseva, T.A. Vinogradova, I.I. Marakhova, 2007, published in Biologicheskie Membrany, 2007, Vol. 24, No. 3, pp. 226–234.

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Epifantseva, I.V., Vinogradova, T.A. & Marakhova, I.I. Ouabain-dependent activation of protein kinase ERK1/2 and the transcription factor STAT3 in carcinoma A431 cells. Biochem. Moscow Suppl. Ser. A 1, 130–137 (2007). https://doi.org/10.1134/S1990747807020055

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  • DOI: https://doi.org/10.1134/S1990747807020055

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