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Cell Motility and Cytoskeleton Structure under the Influence of the Small GTPase RhoA Activator and Inhibitor during Replicative Senescence Process in the MSC Line Derived from the Eyelid Skin of Adult Donor

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Abstract

The widespread use of human MSCs in biomedical technologies and the important role of cell migration in biomedical processes lead to the need to deepen fundamental researches of these cells’ motility during replicative senescence (RS). In this work, we performed a comparative analysis of the structure of the actin cytoskeleton and characteristics of the DF-2 cells’ motility with the presence of an activator (LPA) or an inhibitor (Y-27632) of the small GTPase RhoA at different stages of RS. The following results were obtained: 1) it was shown the presence of RS during long-term cultivation (8–28 passages) of control cells; 2) at the late stage of RS, the presence of 10 ng/mL LPA for 24 h did not cause changes in the structure of the actin cytoskeleton, while the inhibitor of the small GTPase RhoA activity contributed to a significant decrease in the number of stress fibers; 3) changes in the speed and sinuosity of cell movements in the presence of LPA or Y-27632 depend on the stage of RS: in particular, LPA reduces, and Y-27632 increases the mean speed of cell movement at passages 21 and 28 compared with the control, and at passage 8 these agents do not affect cell speed. The results seem to indicate an increase in the activity of RhoA-associated signaling pathways during RS.

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Funding

The work was carried out within the framework of the State Assignment (no. AAAA-A19-119020-190093-9) of the Institute of Cytology of the Russian Academy of Sciences and was supported by the Ministry of Science and Higher Education of the Russian Federation under project 15.BRK.21.0011 (Agreement no. 075-15-2021-1063).

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  1. Institute of Cytology Russian Academy of Sciences, 194064, St. Petersburg, Russia

    D. E. Bobkov, A. V. Polyanskaya, A. S. Musorina, E. V. Lomert & G. G. Poljanskaya

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  1. D. E. Bobkov
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  2. A. V. Polyanskaya
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  3. A. S. Musorina
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  4. E. V. Lomert
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Correspondence to D. E. Bobkov.

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Accepted abbreviations: MSC—mesenchymal stem cells; RS— replicative senescence; LPA—lysophosphatidic acid.

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Bobkov, D.E., Polyanskaya, A.V., Musorina, A.S. et al. Cell Motility and Cytoskeleton Structure under the Influence of the Small GTPase RhoA Activator and Inhibitor during Replicative Senescence Process in the MSC Line Derived from the Eyelid Skin of Adult Donor. Cell Tiss. Biol. 17, 56–66 (2023). https://doi.org/10.1134/S1990519X23010029

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