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The effect of quercetin on hepatic differentiation of human adipose-derived mesenchymal stem cells

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Abstract

Human adipose-derived mesenchymal stem cells (MSCs) can be stimulated to differentiate into hepatic cells. MSC differentiation was induced by fibroblast growth factor-4, hepatocyte growth factor, oncostatin M, and dexamethasone. The influence of quercetin on MSC hepatic differentiation in culture was assayed, and 1 or 10 μmole/L quercetin added into the induction medium enhanced the manifestation of MSC hepatic differentiation. Urea secretion, cytokeratin 19 expression, and α-fetoprotein synthesis were increased. Quercetin modulated CYP1A–cytochrome P450 activity in the differentiated cells. MSCs differentiated in the presence of quercetin exhibited higher viability and resistance to oxidative stress.

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Abbreviations

MSCs:

mesenchymal stem cells

AD:

adipose tissue

TBHP:

tert butyl hydroperoxide

αFP:

α-fetoprotein

CK19:

cytokeratin 19

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Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Minsk, 220072, Belarus

    S. V. Pinchuk, I. B. Vasilevich, Z. B. Kvacheva & I. D. Volotovski

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  1. S. V. Pinchuk
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  2. I. B. Vasilevich
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  3. Z. B. Kvacheva
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  4. I. D. Volotovski
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Correspondence to S. V. Pinchuk.

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Original Russian Text © S.V. Pinchuk, I.B. Vasilevich, Z.B. Kvacheva, I.D. Volotovski, 2016, published in Tsitologiya, 2016, Vol. 58, No. 7, pp. 517–525.

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Pinchuk, S.V., Vasilevich, I.B., Kvacheva, Z.B. et al. The effect of quercetin on hepatic differentiation of human adipose-derived mesenchymal stem cells. Cell Tiss. Biol. 10, 357–364 (2016). https://doi.org/10.1134/S1990519X16050102

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  • DOI: https://doi.org/10.1134/S1990519X16050102

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