Abstract
Electron microscopic histochemistry based upon the oxidative polymerization of 3,3′-diaminobenzidine (DAB) was applied to identify cytochrome c oxidase activity. We found that the incubation of isolated small pieces of cardiac tissue over 72 h under hypoxic conditions caused changes in the mitochondrial ultrastructure and disorders in the functional activity of mitochondria, particularly in the IV complex of respirator chain. Small, electron-dense mitochondria appeared inside electron-light mitochondria (“mitochondria inside mitochondria”) stained positively for cytochrome c oxidase activity along the full length of crista. The results are discussed in connection with the concept of intracellular regeneration and mitochondria structural transformations during mitoptosis.
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Abbreviations
- DAB:
-
3,3′-diaminobenzidine
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Original Russian Text © V.B. Saprunova, I.M. Solodovnikova, L.E. Bakeeva, 2008, published in Tsitologiya, Vol. 50, No. 2, 2008.
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Saprunova, V.B., Solodovnikova, I.M. & Bakeeva, L.E. Cytochrome c oxidase activity in mitochondria of cardiomyocytes from isolated cardiac tissue incubated under long-term hypoxic conditions. Cell Tiss. Biol. 2, 153–157 (2008). https://doi.org/10.1134/S1990519X08020089
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DOI: https://doi.org/10.1134/S1990519X08020089