Skip to main content
Log in

Effect of anionic-lipid-molecule geometry on the structure and properties of liposome-polycation complexes

  • Published:
Polymer Science Series C Aims and scope Submit manuscript

Abstract

The influence of anionic amphiphilic compounds that have different geometric shapes and become incorporated into bilayers on the polycation-mixed liposome interaction and the structure and properties of the resulting complexes is analyzed. Phosphatidylserine, cardiolipin, and sodium dodecyl phosphate are used as anionic lipids, and poly(N-ethyl-4-vinylpyridinium bromide) and polylysine are used as polycations. Polycation adsorption on the surfaces of all examined types of liposomes is accompanied by the neutralization of their charge, an increase in the size of particles of the systems, and quenching of fluorescence labels. Liposomes whose membranes contain incorporated cylindrical phosphatidylserine molecules retain their integrity during contact with polycations. The resulting complexes quantitatively dissociate into initial components during an increase in the salt concentration in the surrounding solution. In the case of liposomes with asymmetric anionic lipids, that is, cardiolipin and sodium dodecyl phosphate, the conditions of retaining the membrane integrity and reversible complexation are fulfilled only at relatively low molar fractions of both lipids. The obtained data witness the decisive effect of the geometry of anionic lipid molecules on the stability of complexes formed from mixed liposomes and polycations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. A. D. Bangham, M. M. Standish, and J. C. Watkins, J. Mol. Biol. 13, 238 (1963).

    Article  Google Scholar 

  2. M. M. Mandy, J. Appl. Res. 7, 69 (2007).

    Google Scholar 

  3. R. J. Lee and L. Huang, J. Biol. Chem. 271, 8481 (1996).

    Article  CAS  Google Scholar 

  4. Liposomes: A Practical Approach, Ed. by V. Torchilin and V. Weissig (Oxford Univ. Press, Oxford, 2003).

    Google Scholar 

  5. B. A. Howell and A. Chauhan, Langmuir 25, 12056 (2009).

    Article  CAS  Google Scholar 

  6. V. A. Kabanov, Polymer Science, Ser. A 46, (2004) [Vysokomol. Soedin., Ser. A 46, 759 (2004)].

    Google Scholar 

  7. I. Henriksen, G. Smistad, and J. Karlsen, Int. J. Pharm. 101, 227 (1994).

    Article  CAS  Google Scholar 

  8. J. F. Gomes, A. Rank, A. Kronenberg, et al., Langmuir 25, 6793 (2009).

    Article  CAS  Google Scholar 

  9. F. Bordi, S. Sennato, and D. Truzzolillo, J. Phys.: Condens. Matter 21, 203102 (2009).

    Article  CAS  Google Scholar 

  10. A. A. Yaroslavov, A. A. Efimova, V. I. Lobyshev, and V. A. Kabanov, Biochim. Biophys. Acta 14, 1560 (2002).

    Google Scholar 

  11. G. G. Hammes and S. E. Schullery, Biochemistry 9, 2555 (1970).

    Article  CAS  Google Scholar 

  12. C. M. Franzin and P. M. Macdonald, Biophys J. 81, 3346 (2001).

    Article  CAS  Google Scholar 

  13. D. Carrier and M. Pezolet, Biochemistry 25, 4167 (1986).

    Article  CAS  Google Scholar 

  14. A. A. Yaroslavov, E. A. Kiseliova, O. Yu. Udalykh, and V. A. Kabanov, Langmuir 14, 5160 (1998).

    Article  CAS  Google Scholar 

  15. R. M. Fuoss and U. P. Strauss, J. Polym. Sci. 3, 602 (1948).

    Article  CAS  Google Scholar 

  16. A. A. Yaroslavov, O. Ye. Kuchenkova, I. B. Okuneva, et al., Biochim. Biophys. Acta 1611, 44 (2003).

    Article  CAS  Google Scholar 

  17. A. A. Yaroslavov, A. A. Rakhnyanskaya, E. G. Yaroslavova, et al., Adv. Colloid Interface Sci. 142, 43 (2008).

    Article  CAS  Google Scholar 

  18. Advances in Planar Bilayers and Liposomes, Ed. by A. Ottova-Leitmannova and H. Tien (Elsevier, New York, 2005).

    Google Scholar 

  19. V. A. Izumrudov, S. I. Kargov, M. V. Zhiryakova, et al., Biopolymers 35, 523 (1995).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A. A. Efimova.

Additional information

Original Russian Text © A.A. Efimova, A.V. Sybachin, A.A. Yaroslavov, 2011, published in Vysokomolekulyarnye Soedineniya, Ser. C, 2011, Vol. 53, No. 7, pp. 1261–1269.

This work was supported by the Russian Foundation for Basic Research, project nos. 08-03-00744 and 09-03-12336.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Efimova, A.A., Sybachin, A.V. & Yaroslavov, A.A. Effect of anionic-lipid-molecule geometry on the structure and properties of liposome-polycation complexes. Polym. Sci. Ser. C 53, 89–96 (2011). https://doi.org/10.1134/S1811238211040011

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1811238211040011

Keywords

Navigation