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Inhibition of Liver Cancer Cell Growth by Triethylsilyl Resveratrol through Targeting Phosphoinositide-3 Kinase Pathway

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Liver cancer is one of the commonly detected malignancy and third highest cause of deaths caused by cancer worldwide. The current study investigated the effect of triethylsilyl resveratrol on HuH7 and hep3B cell proliferation and explored the underlying mechanism. The viability of HuH7 and hep3B cells was suppressed to 23 and 18%, respectively on exposure to 2 µM triethylsilyl resveratrol for 72 h. Triethylsilyl resveratrol treatment of HuH7 cells led to a prominent increase in expression of Atg-5, LC3B-II and Beclin proteins. In triethylsilyl resveratrol treated HuH7 cells expression of activated PI3K and Akt proteins showed a prominent decrease compared to the control cells. Moreover, p-mTOR protein expression was also suppressed in HuH7 cells on treatment with 2 µM triethylsilyl resveratrol. In triethylsilyl resveratrol treated cells a marked increase in ERK1/2 phosphorylation was observed compared to the control cells. Treatment with 2 µM triethylsilyl resveratrol for 72 h led to a significant (p < 0.05) increase in GFP-LC3B labelling in HuH7 cells compared to the control cells. Thus, triethylsilyl resveratrol reduced liver cancer cell viability through increase expression of proteins associated with autophagy. Moreover, it increased p-ERK1/2 expression and targeted activation of PI3K/Akt protein in HuH7 cells. Therefore, triethylsilyl resveratrol may be studied further as a promising therapeutic agent for treatment of liver cancer.

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Authors and Affiliations

  1. Department of General Surgery (Hepatobiliary, Gastrointestinal), The People’s Hospital of Shouguang, 262700, Shouguang City, Shandong Province, China

    Xiaolin Hu

  2. Department of Hepatobiliary Surgery, People’s Hospital of He Chuan Chong Qing, No. 1366, Hechuan District, 401520, Chongqing, China

    Ji Li

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  1. Xiaolin Hu
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  2. Ji Li
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Correspondence to Ji Li.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Hu, X., Li, J. Inhibition of Liver Cancer Cell Growth by Triethylsilyl Resveratrol through Targeting Phosphoinositide-3 Kinase Pathway. Dokl Biochem Biophys 501, 449–453 (2021). https://doi.org/10.1134/S1607672921060065

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