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The Differential Expression of Adhesion Molecule and Extracellular Matrix Genes in Mesenchymal Stromal Cells after Interaction with Cord Blood Hematopoietic Progenitors

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

The dynamics of the expression of genes encoding adhesion molecules, molecules of the connective tissue matrix, and its remodeling enzymes was studied in multipotent mesenchymal stromal cells (MSCs) from human adipose tissue after interaction with cord blood hematopoietic progenitors (HSPCs). An upregulation of ICAM1 and VCAM1, directly proportional to the coculture time (24–72 h), was found. After 72 h of culturing, a downregulation of the genes encoding the majority of matrix molecules (SPP1; COL6A2,7A1; MMP1,3; TIMP1,3; and HAS1) and cell-matrix adhesion molecules (ITGs) was revealed. The detected changes may ensure the realization of the stromal MSC function due to improvement of adhesion and transmigration of HSPCs into the subcellular space.

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, 123007, Russia

    L. B. Buravkova, E. R. Andreeva, M. V. Lobanova, E. V. Cotnezova & A. I. Grigoriev

Authors
  1. L. B. Buravkova
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  2. E. R. Andreeva
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  3. M. V. Lobanova
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  4. E. V. Cotnezova
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  5. A. I. Grigoriev
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Corresponding author

Correspondence to L. B. Buravkova.

Additional information

Original Russian Text © L.B. Buravkova, E.R. Andreeva, M.V. Lobanova, E.V. Cotnezova, A.I. Grigoriev, 2018, published in Doklady Akademii Nauk, 2018, Vol. 479, No. 2, pp. 216–218.

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Buravkova, L.B., Andreeva, E.R., Lobanova, M.V. et al. The Differential Expression of Adhesion Molecule and Extracellular Matrix Genes in Mesenchymal Stromal Cells after Interaction with Cord Blood Hematopoietic Progenitors. Dokl Biochem Biophys 479, 69–71 (2018). https://doi.org/10.1134/S1607672918020047

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  • DOI: https://doi.org/10.1134/S1607672918020047

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