Abstract
This is the first report performing the whole genome SNP scanning of snow sheep (Ovis nivicola). Samples of snow sheep (n = 18) collected in six different regions of the Republic of Sakha (Yakutia) from 64° to 71° N. For SNP genotyping, we applied Ovine 50K SNP BeadChip (Illumina, United States), designed for domestic sheep. The total number of genotyped SNPs (call rate 90%) was 47796 (88.1% of total SNPs), wherein 1006 SNPs were polymorphic (2.1%). Principal component analysis (PCA) showed the clear differentiation within the species O. nivicola: studied individuals were distributed among five distinct arrays corresponding to the geographical locations of sampling points. Our results demonstrate that the DNA chip designed for domestic sheep can be successfully used to study the allele pool and the genetic structure of snow sheep populations.
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Bunch, T.D., Wu, C., Zhang, Y.P., and Wang, S., Phylogenetic analysis of snow sheep (Ovis nivicola) and closely related taxa, J. Hered., 2006, vol. 97, no. 1, pp. 21–30.
Miller, J.M., Poissant, J., Kijas, J.W., and Coltman, D.W., The International Sheep Genomics Consortium (2011). A genome-wide set of SNPs detects population substructure and long-range linkage disequilibrium in wild sheep, Mol. Ecol. Resources, 2011, vol. 11, no. 2, pp. 314–322.
Zheleznov-Chukotskii, N.K., Project OVIS-ZH-CH “Conservation and Accruement of Snow Sheep (Ovis nivicola Eschscholtz, 1829) in Russia 2007–2017,” Vestn. Okhotoved., 2007, vol. 4, no. 3, pp. 285–316.
Okhlopkov, I.M. and Krivoshapkin, A.A., Aerial census of snow sheep abundance in the Verkhoyansk Range area. Final Report, 2010.
Krivoshapkin, A.A. and Yakovlev, F.G., Snezhnyi Baran Verkhoyan’ya (Snow Sheep of Verkhoyansk Area., Yakutsk: Sakhapoligrafizdat, 1999.
Slate, J., Gratten, J., Beraldi, D., Stapley, J., Hale, M., and Pemberton, J.M., Gene mapping in the wild with SNPs: guidelines and future directions, Genetics, 2009, vol. 136, pp. 97–107.
Seeb, J.E., Carvalho, G., Hauser, L., Naish, K., Roberts, S., and Seeb, L.W., Single-nucleotide polymorphism (SNP) discovery and applications of SNP genotyping in nonmodel organisms, Mol. Ecol. Resources, 2011, vol. 11, no. 1, pp. 1–8.
Chang, C.C., Chow, C.C., Cam Tellier, L., Vattikuti, S., Purcell, S.M., and Lee, J.J., Second-generation plink: rising to the challenge of larger and richer datasets, Giga Science, 2015, vol. 4, no. 7, pp. 1–16.
Purcell, S., Neale, B., Todd-Brown, K., Thomas, L., Ferreira, M.A.R., Bender, D., Maller, J., Sklar, P., de Bakker, P.I.W., Daly, M.J., and Sham, P.C., PLINK: A tool set for whole-genome association and population-based linkage analyses, Am. J. Hum. Genet., 2007, vol. 81, pp. 559–575.
Pritchard, J.K., Stephens, M., and Donnelly, P., Inference of population structure using multilocus genotype data, Genetics, 2000, vol. 155, pp. 945–959.
Dent, E.A., von Holdt, B.M., and Bridgett, M., Structure harvester: a website and program for visualizing structure output and implementing the Evanno method, Conserv. Genet. Resources, 2012, vol. 4, no. 2, pp. 359–361.
Evanno, G., Regnaut, S., and Goudet, J., Detecting the number of clusters of individuals using the software structure: a simulation study, Mol. Ecol., 2005, vol. 14, pp. 2611–2620.
Weir, B.S. and Cockerham, C.C., Estimating F-statistics for the analysis of population structure, Evolution, 1984, vol. 38, no. 6, pp. 1358–1370.
Revin, Yu.V., Sopin, L.V., and Zheleznov, N.K., Snezhnyi baran (Snow Sheep., Novosibirsk: Nauka, 1998.
Mlekopitayushchie Yakutii (Mammals of Yakutia), Tavrovskii, V.A., Ed., Moscow: Nauka, 1971, pp. 590–608.
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Original Russian Text © T.E. Deniskova, I.M. Okhlopkov, A.A. Sermyagin, E.A. Gladyr’, V.A. Bagirov, J. Sölkner, N.V. Mamaev, G. Brem, N.A. Zinov’eva, 2016, published in Doklady Akademii Nauk, 2016, Vol. 469, No. 5, pp. 621–626.
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Deniskova, T.E., Okhlopkov, I.M., Sermyagin, A.A. et al. Whole genome SNP scanning of snow sheep (Ovis nivicola). Dokl Biochem Biophys 469, 288–293 (2016). https://doi.org/10.1134/S1607672916040141
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DOI: https://doi.org/10.1134/S1607672916040141