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Expression and purification of an engineered human endothelin receptor B in a monomeric form

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.

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Authors and Affiliations

  1. Moscow Institute of Physics and Technology (State University), Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700, Russia

    A. V. Mishin, A. P. Luginina, A. P. Potapenko, V. I. Borshchevskiy, I. S. Okhrimenko, V. I. Gordeliy & V. G. Cherezov

  2. Bridge Institute, Department of Chemistry, University of Southern California, Los Angeles, California, 90089, USA

    V. Katritch & V. G. Cherezov

  3. The European Molecular Biology Laboratory (EMBL), Grenoble Cedex 9, 38042, France

    E. Edelweiss

  4. CNRS, IBS, Grenoble, 38044, France

    E. Edelweiss & V. I. Gordeliy

  5. Universite Grenoble Alpes, IBS, Grenoble, 38044, France

    V. I. Gordeliy

  6. CEA, IBS, Grenoble, 38044, France

    V. I. Gordeliy

  7. Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics, Research Centre Juelich, Juelich, 52425, Germany

    V. I. Borshchevskiy & V. I. Gordeliy

Authors
  1. A. V. Mishin
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  2. A. P. Luginina
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  3. A. P. Potapenko
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  4. V. I. Borshchevskiy
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  5. V. Katritch
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  6. E. Edelweiss
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  7. I. S. Okhrimenko
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  8. V. I. Gordeliy
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  9. V. G. Cherezov
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Corresponding author

Correspondence to A. V. Mishin.

Additional information

Original Russian Text © A.V. Mishin, A.P. Luginina, A.P. Potapenko, V.I. Borshchevskiy, V. Katritch, E. Edelweiss, I.S. Okhrimenko, V.I. Gordeliy, V.G. Cherezov, 2016, published in Doklady Akademii Nauk, 2016, Vol. 467, No. 6, pp. 725–729.

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Mishin, A.V., Luginina, A.P., Potapenko, A.P. et al. Expression and purification of an engineered human endothelin receptor B in a monomeric form. Dokl Biochem Biophys 467, 157–161 (2016). https://doi.org/10.1134/S1607672916020216

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  • DOI: https://doi.org/10.1134/S1607672916020216

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