Skip to main content
SpringerLink
Log in

Production of Recombinant Allergens Phl p 1 and Amb a 1 for Detection of Class E Immunoglobulins

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract

Recombinant major allergens Phl p 1 from meadow timothy (Phleum pratense) and Amb a 1 from ragweed (Ambrosia artemisiifolia) were obtained in E. coli cells. Allergen purification procedures were developed using anion exchange and affinity chromatography, from which we obtain 10 mg Phl p 1 and 7.5 mg Amb a 1 from 1 L of culture medium with an electrophoretic purity of at least 90%. To analyze the ability of IgE antibodies to bind Phl p 1 and Amb a 1, sera obtained from people with IgE to various respiratory allergens, identified by the RIDA method, were used. Part of the sera bound Phl p 1 or Amb a 1, which confirmed the immunoreactivity of the obtained recombinant proteins. Antibody titers to Amb a 1 correlated with RIDA data on sensitivity to a mixture of herbs and rye pollen. Antibody titers to Phl p 1 did not correlate with RIDA data. Of the 20 positive sera selected, 73 and 89% of the sera recognizing Phl p 1 or Amb a 1, respectively, had antibodies to the cross-reactive alder/birch/hazel allergen group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Haahtela, T., Holgate, S., Pawankar, R., Akdis, C.A., Benjaponpitak, S., Caraballo, L., Demain, J., Portnoy, J., and von Hertzen, L., World Allergy Organization, 2013, pp. 1–13. https://doi.org/10.1186/1939-4551-6-3

  2. Andersson, K. and Lidholm, J., Int. Arch. Allergy Immunol., 2003, vol. 130, pp. 87–107. https://doi.org/10.1159/000069013

    Article  CAS  PubMed  Google Scholar 

  3. Bousquet, P.-J., Chinn, S., Janson, C., Kogevinas, M., Burney, P., and Jarvis, D., Allergy, 2007, vol. 62, pp. 301–309. https://doi.org/10.1111/j.1398-9995.2006.01293.x

    Article  PubMed  Google Scholar 

  4. Focke, M., Mahler, V., Ball, T., Sperr, W.R., Majlesi, Y., Valent, P., Kraft, D., and Valenta, R., FASEB J., 2001, vol. 15, pp. 2042–2044. https://doi.org/10.1096/fj.01-0016fje

    Article  CAS  PubMed  Google Scholar 

  5. Hatzler, L., Panetta, V., Lau, S., Wagner, P., Bergmann, R.L., Illi, S., Bergmann, K.E., Keil, T., Hofmaier, S., Rohrbach, A., Bauer, C.P., Hoffman, U., Forster, J., Zepp, F., Schuster, A., Wahn, U., and Matricardi, P.M., J. Allergy Clin. Immunol., 2012, vol. 130, pp. 894–901, e5. https://doi.org/10.1016/j.jaci.2012.05.053

  6. Storkey, J., Stratonovitch, P., Chapman, D.S., Vidotto, F., and Semenov, M.A., PLoS One, 2014, vol. 9, e88156. https://doi.org/10.1371/journal.pone.0088156

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Smith, M., Cecchi, L., Skjoth, C.A., Karrer, G., and Sikoparija, B., Environ. Int., 2013, vol. 61, pp. 115–126. https://doi.org/10.1016/j.envint.2013.08.005

    Article  CAS  PubMed  Google Scholar 

  8. Suck, R., Kamionka, T., Schaffer, B., Wahl, R., Nandy, A., Weber, B., Petersen, A., Birner-Grunberger, R., Siva Charan, D.V., Keller, W., Fiebig, H., and Cromwell, O., Biochim. Biophys. Acta, 2006, vol. 1764, pp. 1701–1709. https://doi.org/10.1016/j.bbapap.2006.09.015

    Article  CAS  PubMed  Google Scholar 

  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., J. Biol. Chem., 1951, vol. 193, pp. 265–275.

    CAS  PubMed  Google Scholar 

  10. Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  Google Scholar 

  11. Suphioglu, C., Singh, M.B., Taylor, P., Bellomo, R., Holmes, P., Puy, R., and Knox, R.B., Lancet, 1992, vol. 339, pp. 569–572. https://doi.org/10.1016/0140-6736(92)90864-y

    Article  CAS  PubMed  Google Scholar 

  12. Laffer, S., Valenta, R., Vrtala, S., Susani, M., Van Ree, R., Kraft, D., Scheiner, O., and Duchêne, M., J. Allergy Clin. Immunol., 1994, vol. 94, pp. 689–698.

    Article  CAS  Google Scholar 

  13. Westritschnig, K., Horak, F., Swoboda, I., Balic, N., Spitzauer, S., Kundi, M., Fiebig, H., Suck, R., Cromwell, O., and Valenta, R., Eur. J. Clin. Invest., 2008, vol. 38, pp. 260–267. https://doi.org/10.1111/j.1365-2362.2008.01938.x

    Article  CAS  PubMed  Google Scholar 

  14. Niederberger, V., Stübner, P., Spitzauer, S., Kraft, D., Valenta, R., Ehrenberger, K., and Horak, F., J. Invest. Dermatol., 2001, vol. 117, pp. 848–851. https://doi.org/10.1046/j.0022-202x.2001.01470.x

    Article  CAS  PubMed  Google Scholar 

  15. Bokanovic, D., Aberer, W., Hemmer, W., Heinemann, A., Komericki, P., Scheffel, J., and Sturm, G.J., Allergy, 2013, vol. 68, pp. 1403–1409. https://doi.org/10.1111/all.12263

    Article  CAS  PubMed  Google Scholar 

  16. Asero, R., Allergy, 2007, vol. 62, pp. 1097–1099. https://doi.org/10.1111/j.1398-9995.2007.01436.x

    Article  CAS  PubMed  Google Scholar 

  17. Oswalt, M.L. and Marshall, G.D., Allergy Asthma Clin. Immunol., 2008, vol. 4, pp. 130–135. https://doi.org/10.1186/1710-1492-4-3-130

    Article  PubMed  PubMed Central  Google Scholar 

  18. King, T.P., Norman, P.S., and Tao, N., Immunochemistry, 1974, vol. 11, pp. 83–92. https://doi.org/10.1016/0019-2791(74)90321-8

    Article  CAS  PubMed  Google Scholar 

  19. Gadermaier, G., Wopfner, N., Wallner, M., Egger, M., Didierlaurent, A., Regl, G., Aberger, F., Lang, R., Ferreira, F., and Hawranek, T., Allergy, 2008, vol. 63, pp. 1543–1549.

    Article  CAS  Google Scholar 

  20. Bordas-Le, Floch, V., Groeme, R., Chabre, H., Baron-Bodo, V., Nony, E., Mascarell, L., and Moingeon, P., Allergy Asthma Res., vol. 15, p. 63. https://doi.org/10.1007/s11882-015-0565-6

  21. Rafnar, T., Griffith, I.J., Kuo, M.C., Bond, J.F., Rogers, B.L., and Klapper, D.G., J. Biol. Chem., 1991, vol. 266, pp. 1229–1236.

    CAS  PubMed  Google Scholar 

  22. Simonova, M.A., Pivovarov, V.D., Ryazantsev, D.Yu., Kostromina, M.A., Muravieva, T.I., Mokronosova, M.A., Khlgatian, S.V., Esipov, R.S., and Zavriev, S.K., Russ. J. Bioorg. Chem., vol. 44, pp. 217–224. https://doi.org/10.1134/S1068162018010168

  23. Romanov, V.P., Kostromina, T.I., Miroshnikov, A.I., and Feofanov, S.A., Russ. J. Bioorg. Chem., vol. 42, pp. 631–637. https://doi.org/10.1134/S1068162016040154

Download references

Author information

Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    O. O. Mikheeva, M. A. Kostromina, D. D. Lykoshin, M. N. Tereshin, S. K. Zavriev, E. V. Svirshchevskaya & R. S. Esipov

  2. Mechnikov Institute of Vaccines and Sera, 105064, Moscow, Russia

    S. V. Khlgatyan

Authors
  1. O. O. Mikheeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Kostromina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. D. Lykoshin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. N. Tereshin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. K. Zavriev
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. V. Svirshchevskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. V. Khlgatyan
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. R. S. Esipov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. S. Esipov.

Ethics declarations

The article contains no studies involving animals as subjects of the study.

Sera were obtained from volunteers who gave their consent in Mechnikov Research Institute for Vaccines and Sera (Moscow, Russia).

Conflict of Interests

The authors declare they have no conflicts of interests.

Additional information

Translated by N. Onishchenko

Corresponding author: phone: +7(495) 336-68-33.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mikheeva, O.O., Kostromina, M.A., Lykoshin, D.D. et al. Production of Recombinant Allergens Phl p 1 and Amb a 1 for Detection of Class E Immunoglobulins. Russ J Bioorg Chem 46, 1221–1228 (2020). https://doi.org/10.1134/S1068162020060199

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162020060199

Keywords:

Search

Navigation