Abstract
A cell wall has been isolated from single-type cells, phloem fibers of flax (Linum usitatissimum L.) being at the stage of the active formation of a thick secondary cell wall. Weakly bound proteins of the cell wall of phloem fibers were extracted and separated, and their mass spectra were recorded. The identification of the proteins and their assignment to a particular cell compartment were performed using a variety of bioinformatics methods. In all, 93 proteins were identified of which many proteins were defined as predicted, putative, and hypothetical. Twenty one proteins were identified as cell-wall proteins. The absence of the marker proteins of primary cell walls such as xyloglucan endotransglycosylase and expansins indirectly confirms the predominance of the secondary cell wall in a sample for protein extraction.
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Abbreviations
- MALDI TOF MS:
-
matrix-assisted laser desorption ionization time-of-flight mass spectrometry
References
Borderies, G., Jamet, E., Lafitte, C., Rossignol, M., Jauneau, A., Boudart, G., Monsarrat, B., Esquerré-Tugayé, M.T., Boudet, A., and Pont-Lezica, R., Electrophoresis, 2003, vol. 24, pp. 3421–3432.
Boudart, G., Jamet, E., Rossignol, M., Lafitte, C., Borderies, G., Jauneau, A., Esquerré-Tugayé, M.-T., and Pont-Lezica, R., Proteomics, 2005, pp. 212–221.
Zhu, J., Chen, S., Alvarez, S., Asirvatham, V.S., Schachtman, D.P., Wu, Y., and Sharp, R.E., Plant Physiol., 2006, vol. 140, pp. 311–325.
Feiz, L., Irshad, M., Pont-Lezica, R.F., Canut, H., and Jamet, E., Plant Methods, 2006, vol. 2, p. 10.
Minic, Z., Jamet, E., Negroni, L., Der Garabedian, P.A., Zivy, M., and Jouanin, L., J. Exp. Bot., 2007, vol. 58, pp. 2503–2512.
Albenne, C., Canut, H., Boudart, G., Zhang, Y., San Clemente, H., Pont-Lezica, R., and Jamet, E., Mol. Plant, 2009, vol. 2, pp. 977–989.
Gorshkova, T. and Morvan, C., Planta, 2006, vol. 223, no. 2, pp. 149–158.
Vander Mijnsbrugge, K., Meyermans, H., Van Montagu, M., Bauw, G., and Boerjan, W., Planta, 2000, vol. 210, pp. 589–598.
Rep, M., Dekker, H.L., Vossen, J.H., de Boer, A.D., Houterman, P.M., Speijer, D., Back, J.W., de Koster, C.G., and Cornelissen, B.J.C., Plant Physiol., 2002, vol. 130, pp. 904–917.
Gion, J.M., Lalanne, C., Le Provost, G., Ferry-Dumazet, H., Paiva, J., Chaumeil, P., Frigerio, J.M., Brach, J., Barré, A., de Daruvar, A., Claverol, S., Bonneu, M., Sommerer, N., Negroni, L., and Plomion, C., Proteomics, 2005, vol. 5, pp. 3731–3751.
Kalluri, U.C., Hurst, G.B., Lankford, P.K., Ranjan, P., and Pelletier, D.A., Proteomics, 2009, vol. 9, pp. 4871–4880.
Hotte, N.S.C. and Deyholos, M.K.A., BMC Plant Biol., 2008, vol. 8, p. 52. doi: 10.1186/1471-2229-8-52.
Kaku, T., Serada, S., Baba, K., Tanaka, F., and Hayashi, T., J. Wood Sci., 2009, vol. 55, pp. 250–257.
Girault, R., His, I., Andeme-Onzighi, C., Driouich, A., and Morvan, C., Planta, 2000, vol. 211, pp. 256–264.
Ageeva, M.V., Amenitskii, S.I., Gorshkova, T.A., Gur’yanov, O.P., Ibragimova, N.N., Mikshina, P.V., Mokshina, N.E., Sal’nikov, V.V., Snegireva, A.V., Chemikosova, S.B., and Chernova, T.E., in Biogenez rastitel’nykh volokon (Biogenesis of Plant Fibers), Gorshkova, T.A., Ed., Moscow: Nauka, 2009.
Gorshkova, T.A., Salnikov, V.V., Pogodina, N.M., Chemikosova, S.B., Yablokova, E.V., Ulanov, A.V., Ageeva, M.V., van Dam, J.E.G., and Lozovaya, V.V., Ann. Bot., 2000, vol. 85, pp. 477–486.
Irshad, M., Canut, H., Borderies, G., Pont-Lezica, R., and Jamet, E., BMC Plant Biol., 2008, vol. 8, p. 94.
Jamet, E., Albenne, C., Boudart, G., Irshad, M., Canut, H., and Pont-Lezica, R.F., Proteomics, 2008, vol. 8, pp. 893–908.
Dainichenko, E.V., Boldyrev, A.N., Barylyuk, K.V., Polyakov, N.B., and Grinkevich, V.A., Russ. J. Bioorg. Chem., 2009, vol. 35, pp. 411–425.
Watson, B.S., Asirvatham, V.S., Wang, L., and Sumner, L.W., Plant Physiol., 2003, vol. 131, pp. 1104–1123.
Blobel, G. and Dobberstein, B.J., Cell Biol., 1975, vol. 67, pp. 835–851.
Min, X.J., J. Proteomics Bioinformatics, 2010, vol. 4, pp. 143–147.
Rose, J.K.C. and Lee, S.-J., Plant Physiol., 2010, vol. 153, pp. 433–436.
Agrawal, G.K., Jwa, N.S., Lebrun, M.H., Job, D., and Rakwal, R., Proteomics, 2010, vol. 10, pp. 799–827.
Kuwabara, C. and Imai, R., J. Plant Biol., 2009, vol. 52, pp. 19–26.
Stotz, H.U., Spence, B., and Wang, Y.J., Plant. Mol. Biol., 2009, vol. 71, pp. 131–143.
Geisler-Lee, J., Geisler, M., Coutinho, P., Segerman, B., Nishikubo, N., Takahashi, J., Aspeborg, H., Djerbi, S., Master, E., Andersson-Gunneras, S., Sundberg, B., Karpinski, S., Teeri, T., Kleczkowski, L., Henrissat, B., and Mellerowicz, E., Plant Physiol., 2006, vol. 140, pp. 946–962.
Gorshkova, T.A., Salnikov, V.V., Chemikosova, S.B., Ageeva, M.V., Pavlencheva, N.V., and van Dam, J.E.G., Ind. Crops Products, 2003, vol. 18, pp. 213–221.
Bradford, M.M., Anal. Biochem., 1976, vol. 72, pp. 248–254.
Laemmli, U.K., Nature, 1970, vol. 227, pp. 680–685.
Fairbanks, G., Steck, T.L., and Wallach, D.F.H., Biochemistry, 1971, vol. 10, pp. 2606–2617.
Goufman, E.I., Moshkovskii, S.A., Tikhonova, O.V., Lokhov, P.G., Zgoda, V.G., Serebryakova, M.V., Toropygin, I.Yu., Vlasova, M.A., Safarova, M.R., Makarov, O.V., and Archakov, A.I., Biochemistry (Moscow), 2006, vol. 71, pp. 354–360.
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Original Russian Text © N.N. Ibragimova, N.E. Mokshina, T.A. Gorshkova, 2012, published in Bioorganicheskaya Khimiya, 2012, Vol. 38, No. 2, pp. 139–148.
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Ibragimova, N.N., Mokshina, N.E. & Gorshkova, T.A. Cell wall proteins of flax phloem fibers. Russ J Bioorg Chem 38, 117–125 (2012). https://doi.org/10.1134/S1068162012020045
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DOI: https://doi.org/10.1134/S1068162012020045