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Biodegradable microcapsules with entrapped DNA for development of new DNA vaccines

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Abstract

In this study an universal method for preparation of biodegradable microcapsules for antigen entrapment was proposed and optimized. The multilayer microcapsules were prepared by layer-by-layer adsorption of various polyelectrolytes (such as alginate, poly-L-lysine, κ-carrageenan, chitosan and dextran derivatives). High entrapment efficiency of protein and plasmid DNA (non less than 90%) was shown. To carry out in vivo tests, a set of microcapsules with entrapped pTKShi plasmid encoding the E2 polypeptide of classical swine fever was prepared. It was shown that an injection of these microcapsules into mice induced an immune response. The highest antibody titers of mouse blood sera were got after immunization by microcapsules based on modified dextran/carrageenan and modified chitosan/carrageenan. The proposed method for antigen entrapment in biodegradable microcapsules could be used for development of encapsulated vaccines of a new generation (DNA-vaccines).

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Abbreviations

Alg:

sodium alginate

ARC:

antigen-representing cell

BSA:

bovine serum albumin

Car:

κ-carrageenan

CH-1 and CH-2(mod):

quaternary ammonium bases of chitosan

CPP:

a classical pig plague virus

MF:

particles, melamine-formaldehyde latex particles

PGA:

polymaleic acid

PLA:

polylactic acid

PLGA:

lactic and maleic acid copolymers

PLL:

poly-L-lysine

PVA-CH:

polyvinyl alcohol-chitosan copolymer

DEAE-dextran:

diethylaminoethyl-dextran

SCH-1,2:

chitosan sulfates

S-dextran:

dextran sulfate

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    O. E. Selina & E. A. Markvicheva

  2. Research Institute of Veterinary Virology and Microbiology, Pokrov, Vladimir oblast, 601120, Russia

    S. Yu. Belov, N. N. Vlasova, V. I. Balysheva & A. I. Churin

  3. University of Agriculture, Szczecin, Poland

    A. Bartkoviak

  4. Queen Mary University, Mile End Road, London, UK

    G. B. Sukhorukov

Authors
  1. O. E. Selina
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  2. S. Yu. Belov
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  3. N. N. Vlasova
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  4. V. I. Balysheva
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  5. A. I. Churin
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  6. A. Bartkoviak
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  7. G. B. Sukhorukov
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  8. E. A. Markvicheva
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Correspondence to O. E. Selina.

Additional information

Original Russian Text © O.E. Selina, S.Yu. Belov, N.N. Vlasova, V.I. Balysheva, A.I. Churin, A.Bartkoviak, G.B. Sukhorukov, E.A. Markvicheva, 2009, published in Bioorganicheskaya Khimiya, 2009, Vol. 35, No. 1, pp. 113–121.

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Selina, O.E., Belov, S.Y., Vlasova, N.N. et al. Biodegradable microcapsules with entrapped DNA for development of new DNA vaccines. Russ J Bioorg Chem 35, 103–110 (2009). https://doi.org/10.1134/S1068162009010130

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  • DOI: https://doi.org/10.1134/S1068162009010130

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