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Transformation of LTP gene into Brassica napus to enhance its resistance to Sclerotinia sclerotiorum

  • Molecular Genetics
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Abstract

Rapeseed (Brassica napus L.) is one of the most important economic crops worldwide, and Sclerotinia sclerotiorum is the most dangerous disease that affects its yield greatly. Lipid transfer protein (LTP) has broad-spectrum anti-bacterial and fungal activities. In this study, B. napus was transformed using Agrobacterium tumefaciens harboring the plasmid-containing LTP gene to study its possible capability of increasing plant’s resistance. First, we optimized the petiole genetic transformation system by adjusting the days of explants, bacterial concentrations, ratio of hormones, and cultivating condition. Second, we obtained 8 positive plants by PGR analysis of T0 generation. The PGR results of T1 generation were positive, indicating that the LTP gene had been integrated into B. napus. Third, T1 transgenic plants inoculated by detached leaves with mycelia of S. sclerotiorum showed better disease resistance than non-transformants. Oxalic acid belongs to secondary metabolites of S. sclerotiorum, and several studies have demonstrated that the resistance of rapeseed to oxalic acid is significantly consistent with its resistance to S. sclerotiorum. The result from the seed germination assay showed that when T1 seeds were exposed to oxalic acid stress, their germination rate was evidently higher than that of non-transformant seeds. In addition, we measured some physiological changes in T1 plants and control plants under oxalic acid stress. The results showed that T1 transgenic plants had lower malondialdehyde (MDA) content, higher super oxide dismutase (SOD), and peroxidase (POD) activities than non-transformants, whereas disease resistance was related to low MDA content and high SOD and POD activities.

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Authors and Affiliations

  1. Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Y. Fan, K. Du, Y. Gao, Y. Kong & Y. Wang

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    C. Chu

  3. Institute of Molecular and Cell Biology, Siberian Branch Russian Academy of Sciences, Novosibirsk, 630090, Russia

    V. Sokolov

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  1. Y. Fan
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  2. K. Du
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  3. Y. Gao
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Correspondence to Y. Wang.

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Fan, Y., Du, K., Gao, Y. et al. Transformation of LTP gene into Brassica napus to enhance its resistance to Sclerotinia sclerotiorum . Russ J Genet 49, 380–387 (2013). https://doi.org/10.1134/S1022795413040042

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  • DOI: https://doi.org/10.1134/S1022795413040042

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