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Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen

  • Plant Genetics
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Abstract

The pBM plasmid, carrying the gene of hepatitis B virus surface antigen (HBsAg) and free of any selection markers of antibiotic or herbicide resistance, was constructed for genetic transformation of plants. A method for screening transformed plant seedlings on nonselective media was developed. Enzyme immunoassay was used for selecting transgenic plants with HBsAg gene among the produced regenerants; this method provides for a high sensitivity detection of HBsAg in plant extracts. Tobacco and tomato transgenic lines synthesizing this antigen at a level of 0.01–0.05% of the total soluble protein were obtained. The achieved level of HBsAg synthesis is sufficient for preclinical trials of the produced plants as a new generation safe edible vaccine. The developed method for selecting transformants can be used for producing safe plants free of selection markers.

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow oblast, 142290, Russia

    E. B. Rukavtsova, A. R. Gayazova, E. N. Chebotareva & Ya. I. Buryanov

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  1. E. B. Rukavtsova
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  2. A. R. Gayazova
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  3. E. N. Chebotareva
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  4. Ya. I. Buryanov
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Correspondence to E. B. Rukavtsova.

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Original Russian Text © E.B. Rukavtsova, A.R. Gayazova, E.N. Chebotareva, Ya.I. Buryanov, 2009, published in Genetika, 2009, Vol. 45, No. 8, pp. 1055–1060.

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Rukavtsova, E.B., Gayazova, A.R., Chebotareva, E.N. et al. Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen. Russ J Genet 45, 924–928 (2009). https://doi.org/10.1134/S1022795409080055

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  • DOI: https://doi.org/10.1134/S1022795409080055

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